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Planta

, Volume 242, Issue 1, pp 353–363 | Cite as

Down-regulation of lipoxygenase gene reduces degradation of carotenoids of golden rice during storage

  • Dipak Gayen
  • Nusrat Ali
  • Sailendra Nath Sarkar
  • Swapan K. Datta
  • Karabi DattaEmail author
Original Article

Abstract

Main conclusion

Down-regulation of lipoxygenase enzyme activity reduces degradation of carotenoids of bio-fortified rice seeds which would be an effective tool to reduce huge post-harvest and economic losses of bio-fortified rice seeds during storage.

Bio-fortified provitamin A-enriched rice line (golden rice) expressing higher amounts of β-carotene in the rice endosperm provides vitamin A for human health. However, it is already reported that degradation of carotenoids during storage is a major problem. The gene responsible for degradation of carotenoids during storage has remained largely unexplored till now. In our previous study, it has been shown that r9-LOX1 gene is responsible for rice seed quality deterioration. In the present study, we attempted to investigate if r9-LOX1 gene has any role in degradation of carotenoids in rice seeds during storage. To establish our hypothesis, the endogenous lipoxygenase (LOX) activity of high-carotenoid golden indica rice seed was silenced by RNAi technology using aleurone layer and embryo-specific Oleosin-18 promoter. To check the storage stability, LOX enzyme down-regulated high-carotenoid T3 transgenic rice seeds were subjected to artificial aging treatment. The results obtained from biochemical assays (MDA, ROS) also indicated that after artificial aging, the deterioration of LOX-RNAi lines was considerably lower compared to β-carotene-enriched transgenic rice which had higher LOX activity in comparison to LOX-RNAi lines. Furthermore, it was also observed by HPLC analysis that down-regulation of LOX gene activity decreases co-oxidation of β-carotene in LOX-RNAi golden rice seeds as compared to the β-carotene-enriched transgenic rice, after artificial aging treatment. Therefore, our study substantially establishes and verifies that LOX is a key enzyme for catalyzing co-oxidation of β-carotene and has a significant role in deterioration of β-carotene levels in the carotenoid-enriched golden rice.

Keywords

RNAi β-carotene Provitamin A Lipoxygenase Post-harvest deterioration Artificial aging 

Abbreviations

LOX

Lipoxygenase

MDA

Malondialdehyde

ROS

Reactive oxygen species

VAD

Vit-A deficiency

DCFDA

2′, 7′-Dichlorofluorescin diacetate

Notes

Acknowledgments

We thankfully acknowledge Department of Biotechnology (DBT), Government of India (Sanction no. BT/01/COE/06/05) and DST-PURSE, University of Calcutta for financial support. We are also grateful to the Leibniz Institute of Plant Genetics and Crop Plant Research, D–06466 Gatersleben, Germany for providing the pIPKb-006 destination vector.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Laboratory of Translational Research on Transgenic Crops, Department of BotanyUniversity of CalcuttaKolkataIndia
  2. 2.Department of Forest Genetics and Plant Physiology, Umeå Plant Science CentreSwedish University of Agricultural SciencesUmeåSweden

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