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Planta

, Volume 241, Issue 6, pp 1497–1508 | Cite as

Generation of reactive oxygen species in thylakoids from senescing flag leaves of the barley varieties Lomerit and Carina

  • Anja Krieger-Liszkay
  • Mirl Trösch
  • Karin Krupinska
Original Article

Abstract

Main conclusion

During senescence, production of reactive oxygen species increased in thylakoids. In two barley varieties, no difference in superoxide production was observed while singlet oxygen production increased only in one variety.

Abstract

During senescence, chlorophyll content decreased and photosynthetic electron transport was inhibited as shown for flag leaves collected from barley varieties Lomerit and Carina grown in the field. Spin trapping electron paramagnetic resonance (EPR) was used to investigate the production of reactive oxygen species in thylakoid membranes during senescence. EPR measurements were performed with specific spin traps to discriminate between singlet oxygen on one hand and reactive oxygen intermediates on the other hand. The results show that the generation of reactive oxygen intermediates increases in both varieties during senescence. Singlet oxygen increased only in the variety cv. Lomerit while it remained constant at a low level in the variety cv. Carina. Measurements in the presence of inhibitors of photosystem II and of the cytochrome b6f complex revealed that in senescing leaves reduction of oxygen at the acceptor side of photosystem I was the major, but not the only source of superoxide anions. This study shows that during senescence the production of individual reactive oxygen species varies in different barley varieties.

Keywords

Spin trapping electron paramagnetic resonance Reactive oxygen species Thylakoids Photosynthetic electron transport Barley 

Abbreviations

Chl

Chlorophyll

DCMU

3-(3,4-Dichlorophenyl)-1,1-dimethyl urea

DNP-INT

2-Iodo-2′,4′,4′-trinitro-3-methyl-6-isopropyl diphenyl ether

EPR

Electron paramagnetic resonance

LHC

Light harvesting complex

PS

Photosystem

4-POBN

α-(4-Pyridyl-1-oxide)-N-tert-butylnitrone

ROS

Reactive oxygen species

SOD

Superoxide dismutase

TEMPD-HCl

2,2,6,6-Tetramethyl-4-piperidone hydrochloride

Notes

Acknowledgments

We thank Rüdiger Stroeh (farm manager of Hohenschulen, CAU, Kiel, Germany) and his co-workers for preparation of field plots. We also thank the early stage researchers Wera Kucharewicz and Aditi Das of the EU Marie Curie project “Croplife” (ITN: PITN-GA-2010-264394) for collecting samples and Luca Boschian for preparation of chloroplasts in 2013. DEPMPO was a kind gift of S. Pietri, Univeristé Aix-Marseille, France. This work was supported by the German Research Foundation (DFG) for financial support (KR1350/13-1, KR1350/14-1).

Supplementary material

425_2015_2274_MOESM1_ESM.pptx (248 kb)
Supplementary material 1 (PPTX 247 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Anja Krieger-Liszkay
    • 1
  • Mirl Trösch
    • 2
  • Karin Krupinska
    • 2
  1. 1.Institute for Integrative Biology of the Cell (I2BC), Commissariat à l’Energie Atomique et aux Energies Alternatives (CEA) Saclay, Institut de Biologie et de Technologie de Saclay, Centre National de la Recherche Scientifique (CNRS)Université Paris-SudGif-sur-Yvette cedexFrance
  2. 2.Institute of BotanyUniversity of KielKielGermany

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