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Planta

, Volume 241, Issue 6, pp 1395–1404 | Cite as

Natural variance in salt tolerance and induction of starch accumulation in duckweeds

  • K. Sowjanya Sree
  • Kai Adelmann
  • Cyrus Garcia
  • Eric Lam
  • Klaus-J. AppenrothEmail author
Original Article

Abstract

Main conclusion

Ten of 34 tested duckweed clones showed relatively higher salt tolerance. Salinity stress induced high level of starch accumulation in these clones, making them potential feedstock candidates for biofuel production.

Duckweeds are promising as a new generation of crop plants that requires minimal input while providing fast biomass production. Two important traits of interest that can impact on the economic viability of this system are their sensitivity to salt and the starch content of the harvested duckweed. We have surveyed 33 strains of duckweed selected from across all 5 genera and amongst 13 species to quantify the natural variance of these traits. We found that there are large ranges of intraspecific variations in salt tolerance, while all species examined accumulated more starch in response to the initial stages of salt stress. However, the magnitude of the change in starch content varied widely between strains. Our results suggest that specific duckweed clones can be cultivated under relatively saline conditions, while increasing salt in the medium before harvesting could be used to increase starch in duckweed biomass for bioethanol production.

Keywords

Biomass production Duckweed Lemnaceae Salt tolerance Starch accumulation 

Abbreviations

ECx

Effect concentration for the inhibition of growth rate at the level of x % (x = 10, 20, 50)

DW

Dry weight

RGR

Relative growth rates

Notes

Acknowledgments

We thank Prof. em. Dr. Elias Landolt (died on 1. 4. 2013) for the discussion on suitable clones for the present study, and Mr. Robert Bell from Australia for providing the starting material for clone no. 9637 (Landoltia punctata). We also acknowledge the support of Ms. Sabrina Zimmer and Mr. Christoph Schmidt. Research on duckweed in the Lam lab is supported in part by the New Jersey Agricultural Experiment Station (Hatch project #12116) and the School of Environmental and Biological Sciences. CG was supported in part by a Research Assistant fellowship by the Aresty Research Center at Rutgers University. KSS acknowledges the support from Science and Engineering Research Board, Government of India through Fast Track Young Scientist project.

Supplementary material

425_2015_2264_MOESM1_ESM.xlsx (17 kb)
Supplementary material Table S1 Inhibition parameters for duckweed clones under the influence of NaCl. Effective concentration for the inhibition of the growth (EC x ) was calculated on the basis of dry weight for the inhibition levels of 10, 20 and 50 % (EC10, EC20, EC50) for the test period of 7 days according to the ISO20079 protocol. The intervals of confidence (95 % level) are given in brackets (XLSX 17 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • K. Sowjanya Sree
    • 1
  • Kai Adelmann
    • 2
  • Cyrus Garcia
    • 3
  • Eric Lam
    • 3
  • Klaus-J. Appenroth
    • 2
    Email author
  1. 1.Amity Institute of Microbial TechnologyAmity UniversityNoidaIndia
  2. 2.Institute of General Botany and Plant PhysiologyUniversity of JenaJenaGermany
  3. 3.The Rutgers Duckweed Stock Cooperative and the Department of Plant Biology and Pathology, RutgersThe State University of New JerseyNew BrunswickUSA

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