Planta

, Volume 241, Issue 6, pp 1313–1324 | Cite as

Physiological role of phenolic biostimulants isolated from brown seaweed Ecklonia maxima on plant growth and development

  • Adeyemi O. Aremu
  • Nqobile A. Masondo
  • Kannan R. R. Rengasamy
  • Stephen O. Amoo
  • Jiří Gruz
  • Ondřej Bíba
  • Michaela Šubrtová
  • Aleš Pěnčík
  • Ondřej Novák
  • Karel Doležal
  • Johannes Van Staden
Original Article

Abstract

Main conclusion

Eckol, a major phenolic compound isolated from brown seaweed significantly enhanced the bulb size and bioactive compounds in greenhouse-grownEucomis autumnalis.

We investigated the effect of eckol and phloroglucinol (PG) (phenolic compounds) isolated from the brown seaweed, Ecklonia maxima (Osbeck) Papenfuss on the growth, phytochemical and auxin content in Eucomis autumnalis (Mill.) Chitt. The model plant is a popular medicinal species with increasing conservation concern. Eckol and PG were tested at 10−5, 10−6 and 10−7 M using soil drench applications. After 4 months, growth parameters, phytochemical and auxin content were recorded. When compared to the control, eckol (10−6 M) significantly improved bulb size, fresh weight and root production while the application of PG (10−6 M) significantly increased the bulb numbers. However, both compounds had no significant stimulatory effect on aerial organs. Bioactive phytochemicals such as p-hydroxybenzoic and ferulic acids were significantly increased in eckol (10−5 M) and PG (10−6 M) treatments, compared to the control. Aerial (1,357 pmol/g DW) and underground (1,474 pmol/g DW) parts of eckol-treated (10−5 M) plants yielded the highest concentration of indole-3-acetic acid. Overall, eckol and PG elicited a significant influence on the growth and physiological response in E. autumnalis. Considering the medicinal importance of E. autumnalis and the increasing strains on its wild populations, these compounds are potential tools to enhance their cultivation and growth.

Keywords

Asparagaceae Auxins Conservation Phaeophyceae Phytohormones Seaweeds 

Abbreviations

IAA

Indole-3-acetic acid

IAAsp

Indole-3-acetyl-l-aspartic acid

IAC

Immunoaffinity chromatography

MRM

Multiple reaction monitoring

MS

Murashige and Skoog (1962) medium

OxIAA

2-Oxindole-3-acetic acid

PG

Phloroglucinol

PGR

Plant growth regulator

PPF

Photosynthetic photon flux

SPE

Solid-phase extraction

UHPLC

Ultra high performance liquid chromatography–tandem mass spectrometry

UV

Ultra-violet

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Adeyemi O. Aremu
    • 1
  • Nqobile A. Masondo
    • 1
  • Kannan R. R. Rengasamy
    • 1
  • Stephen O. Amoo
    • 1
    • 3
  • Jiří Gruz
    • 2
  • Ondřej Bíba
    • 2
  • Michaela Šubrtová
    • 2
  • Aleš Pěnčík
    • 2
  • Ondřej Novák
    • 2
  • Karel Doležal
    • 2
  • Johannes Van Staden
    • 1
  1. 1.Research Centre for Plant Growth and Development, School of Life SciencesUniversity of KwaZulu-NatalScottsvilleSouth Africa
  2. 2.Laboratory of Growth Regulators & Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of SciencePalacký University and Institute of Experimental Botany AS CROlomoucCzech Republic
  3. 3.Vegetable and Ornamental Plant Institute, RoodeplaatAgricultural Research CouncilPretoriaSouth Africa

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