Planta

, Volume 240, Issue 4, pp 729–743 | Cite as

Ion transport in broad bean leaf mesophyll under saline conditions

  • William J. Percey
  • Lana Shabala
  • Michael C. Breadmore
  • Rosanne M. Guijt
  • Jayakumar Bose
  • Sergey Shabala
Original Article

Abstract

Main conclusion

Salt stress reduces the ability of mesophyll tissue to respond to light. Potassium outward rectifying channels are responsible for 84 % of Na+induced potassium efflux from mesophyll cells.

Modulation in ion transport of broad bean (Vicia faba L.) mesophyll to light under increased apoplastic salinity stress was investigated using vibrating ion-selective microelectrodes (the MIFE technique). Increased apoplastic Na+ significantly affected mesophyll cells ability to respond to light by modulating ion transport across their membranes. Elevated apoplastic Na+ also induced a significant K+ efflux from mesophyll tissue. This efflux was mediated predominately by potassium outward rectifying channels (84 %) and the remainder of the efflux was through non-selective cation channels. NaCl treatment resulted in a reduction in photosystem II efficiency in a dose- and time-dependent manner. In particular, reductions in Fv′/Fm′ were linked to K+ homeostasis in the mesophyll tissue. Increased apoplastic Na+ concentrations induced vanadate-sensitive net H+ efflux, presumably mediated by the plasma membrane H+-ATPase. It is concluded that the observed pump’s activation is essential for the maintenance of membrane potential and ion homeostasis in the cytoplasm of mesophyll under salt stress.

Keywords

Flux H+-ATPase Membrane potential Potassium Photosynthesis 

Abbreviations

DMSO

Dimethyl sulfoxide

KOR

Potassium outward rectifying channels

NHX

Sodium/proton exchanger

NSCC

Non-selective cation channels

PCD

Programmed cell death

ROS

Reactive oxygen species

TEA

Tetraethylammonium

Supplementary material

425_2014_2117_MOESM1_ESM.pptx (173 kb)
Suppl. Fig. S1 a Relationship between net H+ efflux and net K+ flux measured from leaf mesophyll from 15 min after treatment with 100 mM NaCl (a) and 20 mM NaCl (b). One (of six) representative examples is shown for each treatmentSuppl. Fig. S2 Rhythmical net H+ flux response to light/dark fluctuation measured from mesophyll tissues exposed to a range of salinities for 72 h. One (of 6-8) typical examples is shownSuppl. Fig. S3 Rhythmical net K+ flux response to light/dark fluctuation measured from mesophyll tissues exposed to a range of salinities for 72 h. One (of 6-8) typical examples is shown (PPTX 172 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • William J. Percey
    • 1
  • Lana Shabala
    • 1
  • Michael C. Breadmore
    • 2
  • Rosanne M. Guijt
    • 3
  • Jayakumar Bose
    • 1
  • Sergey Shabala
    • 1
  1. 1.School of Land and FoodUniversity of TasmaniaHobartAustralia
  2. 2.Australian Centre for Research on Separation Science (ACROSS), School of ChemistryUniversity of TasmaniaHobartAustralia
  3. 3.School of PharmacyUniversity of TasmaniaHobartAustralia

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