, Volume 239, Issue 6, pp 1363–1373 | Cite as

The fluxes of H2O2 and O2 can be used to evaluate seed germination and vigor of Caragana korshinskii

  • Jiaguo Li
  • Yu Wang
  • Hugh W. Pritchard
  • Xiaofeng Wang
Emerging Technologies


Seed deterioration is detrimental to plant germplasm conservation, and predicting seed germination and vigor with reliability and sensitivity means is urgently needed for practical problems. We investigated the link between hydrogen peroxide (H2O2) flux, oxygen influx and seed vigor of Caragana korshinskii by the non-invasive micro-test technique (NMT). Some related physiological and biochemical changes in seeds were also determined to further explain the changes in the molecular fluxes. The results showed that there was a good linear relationship between germination and H2O2 flux, and that O2 influx was more suitable for assessing seed vigor. H2O2 flux changed relatively little initially, mainly affected by antioxidants (APX, CAT and GSH) and H2O2 content; afterward, the efflux increased more and more rapidly due to high membrane permeability. With the damage of mitochondrial respiration and membrane integrity, O2 influx was gradually reduced. We propose that monitoring H2O2 and O2 fluxes by NMT may be a reliable and sensitive method to evaluate seed germination and vigor.


Seed deterioration Non-invasive micro-test technique H2O2 flux O2 influx Seed vigor 



Phosphate oxygen ratio


Ascorbate peroxidase




Reduced glutathione


Oxidized glutathione


Inner membrane


Non-invasive micro-test technique


Outer membrane


Relative electric conductivity


Reactive oxygen species



This work was supported by National Forest Department 948 Project (Grant No. 2011-4-54) and National Forestry Public Welfare Industry Scientific Research Project (Grant No. 201104024). We thank all researchers for their loyal help during the study. We also thank Xuyue (Beijing) Sci.&Tech.Co., Ltd. for their technical supports, and we declare that the authors have no conflict of interest.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Jiaguo Li
    • 1
  • Yu Wang
    • 1
  • Hugh W. Pritchard
    • 2
  • Xiaofeng Wang
    • 1
  1. 1.National Engineering Laboratory for Tree Breeding, College of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijingChina
  2. 2.Seed Conservation DepartmentRoyal Botanic GardensWest SussexUK

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