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Planta

, Volume 239, Issue 1, pp 97–105 | Cite as

Characterization of a wheat-Psathyrostachys huashanica Keng 4Ns disomic addition line for enhanced tiller numbers and stripe rust resistance

  • Wanli Du
  • Jing Wang
  • Min Lu
  • Shugui Sun
  • Xinhong Chen
  • Jixin Zhao
  • Qunhui Yang
  • Jun Wu
Original Article

Abstract

Exploiting and utilizing excellent gene(s) from wild species has become an essential strategy for wheat improvement. In the disomic addition line 24-6-3, the 4Ns chromosomes from Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) carried valuable tiller and strip rust resistance gene(s), which was selected from the progeny of common wheat cv. 7182 and P. huashanica via embryo culture. Cytology, genomic in situ hybridization (GISH), and EST-STS analyses were used to detect the 4Ns chromosome in wheat background and its homoeologous relationship. Cytological studies demonstrated that 24-6-3 contained 44 chromosomes and formed 22 bivalents during meiotic metaphase I. GISH using P. huashanica genomic DNA as a probe indicated that a pair of Ns-chromosomes with strong hybridization signals had been introduced into 24-6-3. Ten EST-STS markers, i.e., BE404973, BE442811, BE446061, BE446076, BE497324, BE591356, BF473854, BG274986, BQ161513 and CD373484, which were located on the homoeologous group 4 chromosomes of wheat, amplified bands unique to P. huashanica in 24-6-3. This indicated the presence of an introgressed P. huashanica Ns chromosome pair belonging to homoeologous group 4, which we designated the 4Ns disomic addition line. After it was inoculated using mixed races of stripe rust in the adult stages, 24-6-3 expressed high stripe rust resistance, which was possibly derived from its P. huashanica parent. Moreover, its increased number of tillers was probably controlled by gene(s) located in P. huashanica chromosome 4Ns. These high levels of disease resistance and excellent agronomic traits make the 24-6-3 line a promising germplasm for breeding in wheat.

Keywords

Disomic addition line Homoeology Strip rust Molecular cytogenetics Psathyrostachys huashanica Triticum aestivum 

Notes

Acknowledgments

Much appreciated financial support was provided by the Ministry of Science and Technology of the People’s Republic of China (No. 2011AA10010203), the Shaanxi Natural Science Foundation (No.2012JM3001), and the Tang Zhong-Ying Breeding Funding Project of the Northwest A&F University. The authors would like to thank Dr Duncan E. Jackson for useful advice and English language editing of the manuscript.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Wanli Du
    • 1
  • Jing Wang
    • 1
  • Min Lu
    • 1
  • Shugui Sun
    • 1
  • Xinhong Chen
    • 1
  • Jixin Zhao
    • 1
  • Qunhui Yang
    • 1
  • Jun Wu
    • 1
  1. 1.Shaanxi Key Laboratory of Genetic Engineering for Plant Breeding, College of AgronomyNorthwest A&F UniversityYanglingChina

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