Planta

, Volume 238, Issue 5, pp 831–843

γ-Aminobutyric acid (GABA) homeostasis regulates pollen germination and polarized growth in Picea wilsonii

  • Yu Ling
  • Tong Chen
  • Yanping Jing
  • Lusheng Fan
  • Yinglang Wan
  • Jinxing Lin
Original Article

Abstract

γ-Aminobutyric acid (GABA) is a four-carbon non-protein amino acid found in a wide range of organisms. Recently, GABA accumulation has been shown to play a role in the stress response and cell growth in angiosperms. However, the effect of GABA deficiency on pollen tube development remains unclear. Here, we demonstrated that specific concentrations of exogenous GABA stimulated pollen tube growth in Picea wilsonii, while an overdose suppressed pollen tube elongation. The germination percentage of pollen grains and morphological variations in pollen tubes responded in a dose-dependent manner to treatment with 3-mercaptopropionic acid (3-MP), a glutamate decarboxylase inhibitor, while the inhibitory effects could be recovered in calcium-containing medium supplemented with GABA. Using immunofluorescence labeling, we found that the actin cables were disorganized in 3-MP treated cells, followed by the transition of endo/exocytosis activating sites from the apex to the whole tube shank. In addition, variations in the deposition of cell wall components were detected upon labeling with JIM5, JIM7, and aniline blue. Our results demonstrated that calcium-dependent GABA signaling regulates pollen germination and polarized tube growth in P. wilsonii by affecting actin filament patterns, vesicle trafficking, and the configuration and distribution of cell wall components.

Keywords

GABA Pollen tube Calcium Actin filaments Cell wall 

Abbreviations

CLSM

Confocal laser scanning microscopy

GABA

γ-Aminobutyric acid

GABA (A) R

GABA type A receptor

GABARAP

GABA (A) receptor-associated protein

GAD

Glutamate decarboxylase

Glu

Glutamic acid

TCA cycle

Tricarboxylic acid cycle

3-MP

3-mercaptopropionic acid

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Yu Ling
    • 1
  • Tong Chen
    • 2
  • Yanping Jing
    • 1
  • Lusheng Fan
    • 2
  • Yinglang Wan
    • 1
  • Jinxing Lin
    • 1
    • 2
  1. 1.College of Biological Sciences and TechnologyBeijing Forestry UniversityBeijingChina
  2. 2.Key Laboratory of Plant Molecular Physiology, Institute of BotanyChinese Academy of SciencesBeijingChina

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