Planta

, Volume 237, Issue 5, pp 1241–1250 | Cite as

Antimycin A effect on the electron transport in chloroplasts of two Chlamydomonas reinhardtii strains

  • Taras K. Antal
  • Galina P. Kukarskikh
  • Alexander A. Bulychev
  • Esa Tyystjärvi
  • Tatyana Krendeleva
Original Article
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Accepted:

Abstract

The effects of antimycin A on the redox state of plastoquinone and on electron donation to photosystem I (PS I) were studied in sulfur-deprived Chlamydomonas reinhardtii cells of the strains cc406 and 137c. We found that this reagent suppresses cyclic electron flow around PS I in the cc406 strain, whereas this inhibitory effect was completely absent in the 137c strain. In the latter strain, antimycin A induced rapid reduction of plastoquinone in the dark and considerably enhanced the rate of electron donation to P700 + in the dark. Importantly, neither myxothiazol, an inhibitor of mitochondrial respiration, FCCP, a protonophore, nor propyl gallate, an inhibitor of the plastid terminal oxidase, induced such a strong effect like antimycin A. The results indicate that in the chloroplast of the 137c strain, antimycin A has a site of action outside of the machinery of cyclic electron flow.

Keywords

Antimycin A Chlamydomonas Hydrogen Sulfur deprivation 

Abbreviations

AA

Antimycin A

DCMU3

3-(3,4-dichlorophenyl)-1,1-dimethylurea

CEF

Cyclic electron flow around PS I

FQR

Ferredoxin-quinone reductase

Chl

Chlorophyll

ETC

Electron transport chain

FCCP

Carbonylcyanide-p-trifluoromethoxyphenyl-hydrazone

Fd

Ferredoxin

FNR

Ferredoxin-NADP reductase

Mxt

Myxothyazol

MV

Methyl viologen

NDH

NADH dehydrogenase

PPFD

Photosynthetic photon flux density

PQ

Plastoquinon

Pc

Plastocyanin

PTOX

Plastid terminal oxidase

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Notes

Acknowledgments

This study was supported by the Russian Federal Target Programme (8077) (TA, GK, TK). ET thanks European Regional Development Fund (Bio Refine Tech program) for financial support.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Taras K. Antal
    • 1
  • Galina P. Kukarskikh
    • 1
  • Alexander A. Bulychev
    • 1
  • Esa Tyystjärvi
    • 2
  • Tatyana Krendeleva
    • 1
  1. 1.Biological FacultyMoscow State UniversityMoscowRussia
  2. 2.Department of Biochemistry and Food Chemistry Molecular Plant BiologyUniversity of TurkuTurkuFinland

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