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Planta

, Volume 237, Issue 2, pp 509–515 | Cite as

Biparental inheritance of organelles in Pelargonium: evidence for intergenomic recombination of mitochondrial DNA

  • Janina Apitz
  • Andreas WeiheEmail author
  • Frank Pohlheim
  • Thomas Börner
Original Article

Abstract

While uniparental transmission of mtDNA is widespread and dominating in eukaryotes leaving mutation as the major source of genotypic diversity, recently, biparental inheritance of mitochondrial genes has been demonstrated in reciprocal crosses of Pelargonium zonale and P. inquinans. The thereby arising heteroplasmy carries the potential for recombination between mtDNAs of different descent, i.e. between the parental mitochondrial genomes. We have analyzed these Pelargonium hybrids for mitochondrial intergenomic recombination events by examining differences in DNA blot hybridization patterns of the mitochondrial genes atp1 and cob. Further investigation of these genes and their flanking regions using nucleotide sequence polymorphisms and PCR revealed DNA segments in the progeny, which contained both P. zonale and P. inquinans sequences suggesting an intergenomic recombination in hybrids of Pelargonium. This turns Pelargonium into an interesting subject for studies of recombination and evolutionary dynamics of mitochondrial genomes.

Keywords

Biparental inheritance Heteroplasmy Intergenomic recombination Mitochondria Pelargonium Sublimons 

Abbreviation

mt

Mitochondrial

Notes

Acknowledgments

The excellent technical assistance of Cornelia Stock is gratefully acknowledged.

Supplementary material

425_2012_1768_MOESM1_ESM.pdf (2.7 mb)
Supplementary material 1 (PDF 2725 kb)
425_2012_1768_MOESM2_ESM.pdf (2.1 mb)
Supplementary material 2 (PDF 2174 kb)
425_2012_1768_MOESM3_ESM.docx (20 kb)
Supplementary material 3 (DOCX 20 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Janina Apitz
    • 1
  • Andreas Weihe
    • 1
    Email author
  • Frank Pohlheim
    • 2
  • Thomas Börner
    • 1
  1. 1.Institut für BiologieHumboldt-Universität zu BerlinBerlinGermany
  2. 2.KleinmachnowGermany

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