Planta

, Volume 237, Issue 1, pp 15–27 | Cite as

SUI-family genes encode phosphatidylserine synthases and regulate stem development in rice

  • Hengfu Yin
  • Peng Gao
  • Chengwu Liu
  • Jun Yang
  • Zhongchi Liu
  • Da Luo
Original Article

Abstract

In vascular plants, the regulation of stem cell niche determines development of aerial shoot which consists of stems and lateral organs. Intercalary meristem (IM) controls internode elongation in rice and other grasses, however little attention has been paid to the underlying mechanism of stem cell maintenance. Here, we investigated the stem development in rice and showed that the Shortened Uppermost Internode 1 (SUI1) family of genes are pivotal for development of rice stems. We demonstrated that SUI-family genes regulate the development of IM for internode elongation and also the cell expansion of the panicle stem rachis in rice. The SUI-family genes encoded base-exchange types of phosphatidylserine synthases (PSSs), which possessed enzymatic activity in a yeast complementary assay. Overexpression of SUI1 and SUI2 caused outgrowths of internodes during vegetative development, and we showed that expression patterns of Oryza Sativa Homeobox 15 (OSH15) and Histone4 were impaired. Furthermore, genome-wide gene expression analysis revealed that overexpression and RNA knockdown of SUI-family genes affected downstream gene expression related to phospholipid metabolic pathways. Moreover, using Ultra-performance liquid chromatography–quadrupole time of flight-mass spectrometry, we analyzed PS contents in different genetic backgrounds of rice and showed that the quantity of very long chain fatty acids PS is affected by transgene of SUI-family genes. Our study reveals a new mechanism conveyed by the SUI1 pathway and provides evidence to link lipid metabolism with plant stem cell maintenance.

Keywords

Intercalary meristem Internode development Phosphatidylserine synthase Very long chain fatty acids 

Abbreviations

IM

Intercalary meristem

OSH15

Oryza Sativa Homeobox 15

PS

Phosphatidylserine

PSS

Phosphatidylserine synthase

SUII

Shortened Uppermost Internode 1

VLCFA

Very long chain fatty acids

PE

Phosphatidyl ethanolamine

UPLC

Ultra-performance liquid chromatography

Supplementary material

425_2012_1736_MOESM1_ESM.docx (1003 kb)
Supplementary material 1 (DOCX 67 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Hengfu Yin
    • 1
  • Peng Gao
    • 1
  • Chengwu Liu
    • 1
  • Jun Yang
    • 1
  • Zhongchi Liu
    • 2
  • Da Luo
    • 1
  1. 1.National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of SciencesChinese Academy of SciencesShanghaiChina
  2. 2.Department of Cell Biology and Molecular GeneticsUniversity of MarylandMarylandUSA

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