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Planta

, Volume 236, Issue 4, pp 1305–1313 | Cite as

In planta production of a candidate vaccine against bovine papillomavirus type 1

  • Andrew J. LoveEmail author
  • Sean N. Chapman
  • Slavica Matic
  • Emanuela Noris
  • George P. Lomonossoff
  • Michael Taliansky
Original Article

Abstract

Bovine papillomavirus type 1 (BPV-1) is an economically important virus that induces tumourigenic pathologies in horses and cows. Given that the BPV-1 L1 major coat protein can self-assemble into highly immunogenic higher-order structures, we transiently expressed it in Nicotiana benthamiana as a prelude to producing a candidate vaccine. It was found that plant codon optimization of L1 gave higher levels of expression than its non-optimized counterpart. Following protein extraction, we obtained high yields (183 mg/kg fresh weight leaf tissue) of relatively pure L1, which had self-assembled into virus-like particles (VLPs). We found that these VLPs elicited a highly specific and strong immune response, and therefore they may have utility as a potential vaccine. This is the first report demonstrating the viable production of a candidate BPV vaccine protein in plants.

Keywords

Bovine papillomavirus Codon optimization Plant expression Vaccine protein Virus-like particles 

Abbreviations

BPV

Bovine papillomavirus

CPMV

Cowpea mosaic virus

CRPV

Cottontail rabbit papillomavirus

ELISA

Enzyme-linked immunosorbent assay

HPV

Human papillomavirus

Mes

2-Morpholinoethanesulphonic acid

PBS

Phosphate-buffered saline

SDS-PAGE

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis

UTRs

Untranslated regions

VLP

Virus-like particle

Notes

Acknowledgments

The authors would like to thank Prof. Lesley Torrance and Graham Cowan (The James Hutton Institute, Invergowrie, Scotland, UK) for helpful and critical discussions. We would also like to thank Profs. Lubna Nasir and Saveria Campo (Glasgow University, Glasgow, Scotland, UK) for provision of the BPV-1 clone. We are very grateful to Prof. Reinhard Kirnbauer and Dr. Sabine Brandt (University of Vienna Medical School, Vienna, Austria) for the provision of insect cell-produced BPV L1 VLPs. This was funded by the EU PLAPROVA KBBE-2008-227056 grant, and the work of AJL, SNC and MT was also partly funded by the Scottish Government’s Rural and Environmental Science and Analytical Services (RESAS) Division.

Supplementary material

425_2012_1692_MOESM1_ESM.doc (29 kb)
Supplementary material 1 (DOC 29 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Andrew J. Love
    • 1
    Email author
  • Sean N. Chapman
    • 1
  • Slavica Matic
    • 2
  • Emanuela Noris
    • 2
  • George P. Lomonossoff
    • 3
  • Michael Taliansky
    • 1
  1. 1.The James Hutton Institute (Dundee)DundeeUK
  2. 2.Istituto di Virologia VegetaleTurinItaly
  3. 3.Department of Biological ChemistryJohn Innes CentreNorwichUK

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