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Planta

, Volume 236, Issue 5, pp 1405–1417 | Cite as

Construction and analysis of EST libraries of the trans-polyisoprene producing plant, Eucommia ulmoides Oliver

  • Nobuaki Suzuki
  • Hirotaka Uefuji
  • Takashi Nishikawa
  • Yukio Mukai
  • Atsushi Yamashita
  • Masahira Hattori
  • Naotake Ogasawara
  • Takeshi Bamba
  • Ei-ichiro Fukusaki
  • Akio Kobayashi
  • Yoshiyuki Ogata
  • Nozomu Sakurai
  • Hideyuki Suzuki
  • Daisuke Shibata
  • Yoshihisa Nakazawa
Original Article

Abstract

Eucommia ulmoides Oliver is one of a few woody plants capable of producing abundant quantities of trans-polyisoprene rubber in their leaves, barks, and seed coats. One cDNA library each was constructed from its outer stem tissue and inner stem tissue. They comprised a total of 27,752 expressed sequence tags (ESTs) representing 10,520 unigenes made up of 4,302 contigs and 6,218 singletons. Homologues of genes coding for rubber particle membrane proteins that participate in the synthesis of high-molecular poly-isoprene in latex were isolated, as well as those encoding known major latex proteins (MLPs). MLPs extensively shared ESTs, indicating their abundant expression during trans-polyisoprene rubber biosynthesis. The six mevalonate pathway genes which are implicated in the synthesis of isopentenyl diphosphate (IPP), a starting material of poly-isoprene biosynthesis, were isolated, and their role in IPP biosynthesis was confirmed by functional complementation of suitable yeast mutants. Genes encoding five full-length trans-isoprenyl diphosphate synthases were also isolated, and two among those synthesized farnesyl diphosphate from IPP and dimethylallyl diphosphate, an assumed intermediate of rubber biosynthesis. This study should provide a valuable resource for further studies of rubber synthesis in E. ulmoides.

Keywords

Eucommia ulmoides Oliv. EST Hardy rubber tree Isoprenoid Prenyltransferase Trans-polyisoprene 

Abbreviations

DMAPP

Dimethylallyl diphosphate

DXP

Deoxy-xylulose phosphate

FPP

Farnesyl diphosphate

GGPP

Geranylgeranyl diphosphate

HMG-CoA

3-Hydroxy-3-methylglutaryl-CoA

IPP

Isopentenyl diphosphate

IT

Inner stem tissue

MEP

Methylerythritol phosphate

MLP

Major latex protein

OT

Outer stem tissue

REF

Rubber elongation factor

RPMP

Rubber particle membrane protein

SRPP

Small rubber particle protein

TIDS

Trans-isoprenyl diphosphate synthase

Notes

Acknowledgments

This work was partially supported by the New Energy and Industrial Technology Development Organization (NEDO).

Supplementary material

425_2012_1679_MOESM1_ESM.pdf (11 kb)
Supplementary material 1 (PDF 11 kb)
425_2012_1679_MOESM2_ESM.pdf (12 kb)
Supplementary material 2 (PDF 11 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Nobuaki Suzuki
    • 1
  • Hirotaka Uefuji
    • 6
  • Takashi Nishikawa
    • 1
  • Yukio Mukai
    • 2
  • Atsushi Yamashita
    • 3
  • Masahira Hattori
    • 3
  • Naotake Ogasawara
    • 4
  • Takeshi Bamba
    • 1
  • Ei-ichiro Fukusaki
    • 1
  • Akio Kobayashi
    • 1
  • Yoshiyuki Ogata
    • 5
  • Nozomu Sakurai
    • 5
  • Hideyuki Suzuki
    • 5
  • Daisuke Shibata
    • 5
  • Yoshihisa Nakazawa
    • 1
    • 6
  1. 1.Department of Biotechnology, Graduate School of EngineeringOsaka UniversitySuitaJapan
  2. 2.Department of BioscienceNagahama Institute of Bio-Science and TechnologyNagahamaJapan
  3. 3.Kitasato Institute for Life SciencesKitasato UniversitySagamiharaJapan
  4. 4.Department of Information Systems, Graduate School of Information ScienceNara Institute of Science and TechnologyIkomaJapan
  5. 5.Kazusa DNA Research InstituteKisarazuJapan
  6. 6.Technical Research InstituteHitachi Zosen CorporationOsakaJapan

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