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Planta

, Volume 236, Issue 2, pp 343–354 | Cite as

Hormonal changes during flower development in floral tissues of Lilium

Original Article

Abstract

Much effort has been focussed on better understanding the key signals that modulate floral senescence. Although ethylene is one of the most important regulators of floral senescence in several species, Lilium flowers show low sensitivity to ethylene; thus their senescence may be regulated by other hormones. In this study we have examined how (1) endogenous levels of hormones in various floral tissues (outer and inner tepals, androecium and gynoecium) vary throughout flower development, (2) endogenous levels of hormones in such tissues change in cut versus intact flowers at anthesis, and (3) spray applications of abscisic acid and pyrabactin alter flower longevity. Results show that floral tissues behave differently in their hormonal changes during flower development. Cytokinin and auxin levels mostly increased in tepals prior to anthesis and decreased later during senescence. In contrast, levels of abscisic acid increased during senescence, but only in outer tepals and the gynoecium, and during the latest stages. In addition, cut flowers at anthesis differed from intact flowers in the levels of abscisic acid and auxins in outer tepals, salicylic acid in inner tepals, cytokinins, gibberellins and jasmonic acid in the androecium, and abscisic acid and salicylic acid in the gynoecium, thus showing a clear differential response between floral tissues. Furthermore, spray applications of abscisic acid and pyrabactin in combination accelerated the latest stages of tepal senescence, yet only when flower senescence was delayed with Promalin. It is concluded that (1) floral tissues differentially respond in their endogenous variations of hormones during flower development, (2) cut flowers have drastic changes in the hormonal balance not only of outer and inner tepals but also of androecium and gynoecium, and (3) abscisic acid may accelerate the progression of tepal senescence in Lilium.

Keywords

Abscisic acid Cytokinins Floral tissues Flower senescence Hormones Lilium 

Abbreviations

ABA

Abscisic acid

GA4

Gibberellin 4

IAA

Indole-3-acetic acid

JA

Jasmonic acid

PYR

Pyrabactin

SA

Salicylic acid

Z

Zeatin

ZR

Zeatin riboside

Notes

Acknowledgments

We are very grateful to the Serveis Científico-Tècnics and Serveis dels Camps Experimentals (Universitat de Barcelona) for technical assistance. Support for the research was received through the prize ICREA Academia given to S.M.-B., funded by the Generalitat de Catalunya.

Supplementary material

425_2012_1615_MOESM1_ESM.pptx (799 kb)
Supplementary material 1 (PPTX 799 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Departament de Biologia Vegetal, Facultat de BiologiaUniversitat de BarcelonaBarcelonaSpain

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