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Planta

, Volume 236, Issue 1, pp 51–61 | Cite as

The role of CCoAOMT1 and COMT1 in Arabidopsis anthers

  • Christin Fellenberg
  • Maike van Ohlen
  • Vinzenz Handrick
  • Thomas Vogt
Original Article

Abstract

Arabidopsis caffeoyl coenzyme A dependent O-methyltransferase 1 (CCoAOMT1) and caffeic acid O-methyltransferase 1 (COMT1) display a similar substrate profile although with distinct substrate preferences and are considered the key methyltransferases (OMTs) in the biosynthesis of lignin monomers, coniferyl and sinapoylalcohol. Whereas CCoAOMT1 displays a strong preference for caffeoyl coenzyme A, COMT1 preferentially methylates 5-hydroxyferuloyl CoA derivatives and also performs methylation of flavonols with vicinal aromatic dihydroxy groups, such as quercetin. Based on different knockout lines, phenolic profiling, and immunohistochemistry, we present evidence that both enzymes fulfil distinct, yet different tasks in Arabidopsis anthers. CCoAOMT1 besides its role in vascular tissues can be localized to the tapetum of young stamens, contributing to the biosynthesis of spermidine phenylpropanoid conjugates. COMT1, although present in the same organ, is not localized in the tapetum, but in two directly adjacent cells layers, the endothecium and the epidermal layer of stamens. In vivo localization and phenolic profiling of comt1 plants provide evidence that COMT1 neither contributes to the accumulation of spermidine phenylpropanoid conjugates nor to the flavonol glycoside pattern of pollen grains.

Keywords

Arabidopsis O-Methyltransferase Phenylpropanoids Pollen Spermidine Tapetum 

Abbreviations

AtTSM1

Flower bud-specific O-methyltransferase

BCIP/NBT

Bromo-chloro-indolyl phosphate/nitroblue tetrazolium

CCoAOMT

Caffeoyl coenzyme A O-methyltransferase

COMT

Caffeic acid O-methyltransferase

HCAA

Hydroxycinnamic acid amide

OMT

O-Methyltranferase

qPCR

Quantitative real-time polymerase chain reaction

SHT

Spermidine hydroxycinnamic acid transferase

Notes

Acknowledgments

We thank Alain Tissier (IPB, Halle) for critical reading of the manuscript. Financial support by the Deutsche Forschungsgemeinschaft (Vo 719/8-1) is gratefully acknowledged.

Supplementary material

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Supplementary material 1 (DOC 20 kb)
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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Christin Fellenberg
    • 1
  • Maike van Ohlen
    • 1
    • 2
  • Vinzenz Handrick
    • 1
  • Thomas Vogt
    • 1
  1. 1.Department of Cell and Metabolic BiologyLeibniz Institute of Plant BiochemistryHalle (Saale)Germany
  2. 2.Institute of Pharmaceutical BiologyBraunschweig University of TechnologyBrunswickGermany

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