, Volume 235, Issue 6, pp 1221–1237 | Cite as

Regulation of polyamine metabolism and biosynthetic gene expression during olive mature-fruit abscission

Original Article


Exogenous ethylene and some inhibitors of polyamine biosynthesis can induce mature-fruit abscission in olive, which could be associated with decreased nitric oxide production as a signaling molecule. Whether H2O2 also plays a signaling role in mature-fruit abscission is unknown. The possible involvement of H2O2 and polyamine in ethylene-induced mature-fruit abscission was examined in the abscission zone and adjacent cells of two olive cultivars. Endogenous H2O2 showed an increase in the abscission zone during mature-fruit abscission, suggesting that accumulated H2O2 may participate in abscission signaling. On the other hand, we followed the expression of two genes involved in the polyamine biosynthesis pathway during mature-fruit abscission and in response to ethylene or inhibitors of ethylene and polyamine. OeSAMDC1 and OeSPDS1 were expressed differentially within and between the abscission zones of the two cultivars. OeSAMDC1 showed slightly lower expression in association with mature-fruit abscission. Furthermore, our data show that exogenous ethylene or inhibitors of polyamine encourage the free putrescine pool and decrease the soluble-conjugated spermidine, spermine, homospermidine, and cadaverine in the olive abscission zone, while ethylene inhibition by CoCl2 increases these soluble conjugates, but does not affect free putrescine. Although the impact of these treatments on polyamine metabolism depends on the cultivar, the results confirm that the mature-fruit abscission may be accompanied by an inhibition of S-adenosyl methionine decarboxylase activity, and the promotion of putrescine synthesis in olive abscission zone, suggesting that endogenous putrescine may play a complementary role to ethylene in the normal course of mature-fruit abscission.


Abscission zone Ethylene Mature-fruit abscission Polyamine S-Adenosyl methionine decarboxylase Spermidine synthase 



1-Aminocyclopropane-1-carboxylic acid


1-Aminocyclopropane-1-carboxylic acid oxidase


1-Aminocyclopropane-1-carboxylic acid synthase


Arginine decarboxylase


Arbequina cultivar


Abscission zone


Abscission zone and adjacent cells






Confocal laser scanning microscopy


Decarboxylated S-adenosylmethionine


Dichlorodihydrofluorescein diacetate


Diamine oxidase


EIN3-like gene




Fruit-detachment force

HomoSpd sym



Homospermidine synthase






Nitric oxide


Olea europaea ACS 2


Olea europaea EIL 2


Olea europaea SAMDC 1


Olea europaea SPDS 1


Ornithine decarboxylase




Polyamine oxidase


Picual cultivar




Reactive oxygen species




S-Adenosylmethionine decarboxylase




Spermidine synthase




Spermine synthase

Supplementary material

425_2011_1570_MOESM1_ESM.tif (784 kb)
Supplementary material 1 (TIFF 783 kb)
425_2011_1570_MOESM2_ESM.tif (783 kb)
Supplementary material 2 (TIFF 782 kb)


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Department of Plant PhysiologyUniversity of ExtremaduraBadajozSpain

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