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Planta

, Volume 235, Issue 6, pp 1157–1169 | Cite as

bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice

Original Article

Abstract

OsbZIP52/RISBZ5 is a member of the basic leucine zipper (bZIP) transcription factor (TF) family in rice (Oryza sativa) isolated from rice (Zhonghua11) panicles. Expression of the OsbZIP52 gene was strongly induced by low temperature (4°C) but not by drought, PEG, salt, or ABA. The subcellular localization of OsbZIP52-GFP in onion (Allium cepa) epidermis cells revealed that OsbZIP52 is a nuclear localized protein. A transactivation assay in yeast demonstrated that OsbZIP52 functions as a transcriptional activator and can specifically bind to the G-box promoter motif. In a yeast two-hybrid (Y-2-H) experiment, OsbZIP52 was able to form homodimeric complexes. Rice plants overexpressing OsbZIP52 showed significantly increased sensitivity to cold and drought stress. Real-time PCR analysis revealed that some abiotic stress-related genes, such as OsLEA3, OsTPP1, Rab25, gp1 precursor, β-gal, LOC_Os05g11910 and LOC_Os05g39250, were down-regulated in OsbZIP52 overexpression lines. These results suggest that OsbZIP52/RISBZ5 could function as a negative regulator in cold and drought stress environments.

Keywords

Abiotic stress bZIP Transcription factor G-box Transgenic rice (Oryza sativa

Abbreviations

bZIP

Basic leucine zipper

LEA

Rice late embryogenesis abundant protein

TPP

Trehalose-6-phosphate phosphatase

gp1

Glycoprotein 1

RAB

Responsive to abscisic acid

O2

Opaque-2

RITA-1

Rice transcription activator-1

RISBZ

Rice seed b-Zipper

G/HBF1

G-box/H-box binding factors

BZO2H

Basic leucine zipper O2 homolog

Lip19

Low-temperature-induced protein 19

Notes

Acknowledgments

This research was supported by grants from the Agricultural Ministry of China (2011ZX08009-003-002), and the National Natural Science Foundation of China (2010 No. 31071378).

Supplementary material

425_2011_1564_MOESM1_ESM.doc (2 mb)
Suppl. Fig. S1 Seedling development of OsbZIP52 overexpression transgenic rice plants under ABA treatment. ab The seed germination rate and seedling growth of OsbZIP52-overexpression rice lines with and without ABA. c Shoot and root lengths and root number at different ABA concentrations in the WT control (ZH11) and the transgenic rice lines. d Seedlings grown at different concentrations of ABA for 12 days. All the experiments were repeated at least three times (150 seeds for each replicate). ZH11, wild-type Zhonghua11 rice variety. 197-1, -3, -16, -24, -27 are independent transgenic lines of OsbZIP52 (DOC 2055 kb)
425_2011_1564_MOESM2_ESM.doc (334 kb)
Suppl. Fig. S2 OsbZIP52 expression in three rice varieties. a OsbZIP52 expression patterns in different tissues of rice variety Nipponbare. bd Expression of OsbZIP52 in three rice varieties grown at low temperature (4°C) measured by real-time PCR. DAF, days after flowering (DOC 333 kb)
425_2011_1564_MOESM3_ESM.doc (44 kb)
Suppl. Table S1. Primers used in semi-quantitative RT-PCR and real-time PCR analysis and constructs for vectors (DOC 44 kb)
425_2011_1564_MOESM4_ESM.doc (32 kb)
Suppl. Table S2 Cis-acting elements analysis in promoters of stress-regulated genes in OsbZIP52 overexpression lines. (DOC 31 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Key Laboratory of Gene Engineering Drug and Biotechnology, Key Laboratory of Cell Proliferation and Regulation of Ministry of EducationCollege of Life Sciences, Beijing Normal UniversityBeijingPeople’s Republic of China
  2. 2.National Center for Molecular Crop DesignWeiming Kaituo Agriculture Biotech Co., LtdBeijingPeople’s Republic of China

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