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Planta

, Volume 235, Issue 5, pp 955–964 | Cite as

Sucrose synthase in unicellular cyanobacteria and its relationship with salt and hypoxic stress

  • María A. Kolman
  • Leticia L. Torres
  • Mariana L. Martin
  • Graciela L. SalernoEmail author
Original Article

Abstract

Higher plants and cyanobacteria metabolize sucrose (Suc) by a similar set of enzymes. Suc synthase (SuS, A/UDP-glucose: d-fructose 2-α-d-glucosyl transferase) catalyzes a reversible reaction. However, it is in the cleavage of Suc that this enzyme plays an important role in vivo, providing sugar nucleotides for polysaccharide biosynthesis. In cyanobacteria, SuS occurrence has been reported in heterocyst-forming strains, where it was shown to be involved also in nitrogen fixation. We investigated the presence of sequences homologous to SuS-encoding genes (sus) in recently sequenced cyanobacterial genomes. In this work, we show for the first time the presence of SuS in unicellular cyanobacterium strains (Microcystis aeruginosa PCC 7806, Gloebacter violaceus PCC 7421, and Thermosynechococcus elongatus BP-1). After functional characterization of SuS encoding genes, we demonstrated an increase in their transcript levels after a salt treatment or hypoxic stress in M. aeruginosa and G. violaceus cells. Based on phylogenetic analysis and on the presence of sus homologs in the most recently radiated cyanobacterium strains, we propose that sus genes in unicellular cyanobacteria may have been acquired through horizontal gene transfer. Taken together, our data indicate that SuS acquisition by cyanobacteria might be related to open up new ecological niches.

Keywords

Gloeobacter violaceus Hypoxia Microcystis aeruginosa Salt stress Sucrose metabolism Thermosynechococcus elongatus 

Abbreviations

Gv

Gloebacter violaceus

Ma

Microcystis aeruginosa

Suc

Sucrose

SuS

Sucrose synthase

sus, susA

Sucrose synthase encoding gene

Te

Thermosynechoccocus elongatus

Notes

Acknowledgments

We are very thankful to H.G. Pontis for insightful reading of the manuscript and for helpful discussions, and C. Fernández and M. Vidal for technical assistance. This research was funded by Grants from CONICET (PIP 134), Universidad Nacional de Mar del Plata, and FIBA.

Supplementary material

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Supplementary material 1 (PDF 6 kb)
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Supplementary material 2 (PDF 6 kb)
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Supplementary material 3 (PDF 129 kb)
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Supplementary material 4 (PDF 100 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • María A. Kolman
    • 1
  • Leticia L. Torres
    • 1
    • 2
  • Mariana L. Martin
    • 1
  • Graciela L. Salerno
    • 1
    Email author
  1. 1.Centro de Investigaciones Biológicas, CEBB-MdP-INBA, Fundación para Investigaciones Biológicas Aplicadas (FIBA)Mar del PlataArgentina
  2. 2.Departamento de Biología Molecular, Centro de Biología Molecular Severo OchoaUniversidad Autónoma de MadridMadridSpain

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