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Planta

, Volume 235, Issue 4, pp 713–727 | Cite as

OsLIS-L1 encoding a lissencephaly type-1-like protein with WD40 repeats is required for plant height and male gametophyte formation in rice

  • Xinqiang Gao
  • Zhihui Chen
  • Jian Zhang
  • Xingwang Li
  • Guoxing Chen
  • Xianghua Li
  • Changyin WuEmail author
Original Article

Abstract

Although a large number of genes encoding the WD40 motif have been identified as being involved in various developmental processes in Arabidopsis, little is known about the function of these genes in rice (Oryza sativa). Here, we report the cloning and functional characterization of a novel rice gene OsLIS-L1 (Lissencephaly type-1-like 1), which is required for normal fertility and the first internode elongation. OsLIS-L1 encodes a lissencephaly type-1-like protein containing the WD40 motif that is required for brain development in human. SMART algorithm analysis indicated that OsLIS-L1 contains a LIS1 homology (LisH) domain, a C terminus to LisH (CTLH) domain, a five WD40-repeat domain in the middle, and a domain with four WD40 repeats which is homologous to the β subunit of trimeric G-proteins (Gβ). OsLIS-L1 transcript is relatively highly abundant in stem and panicle and has a dynamic expression pattern at different panicle developmental stages. Two independent alleles, designated oslis-l1-1 and oslis-l1-2, exhibited similar abnormal developmental phenotypes, including semi-dwarf, shorter panicle length, and reduced male fertility. Cytological examination confirmed that OsLIS-L1 does not affect the meiosis in pollen mother cells. Compared with wild type, the oslis-l1 mutant had abnormal male gametophyte formation, but anther cell wall and pollen wall development were not affected. Histological analysis revealed that OsLIS-L1 regulates the cell proliferation in the first internode under the panicle. Our results indicate that OsLIS-L1 plays an important role in male gametophyte formation and the first internode elongation in rice.

Keywords

Lissencephaly 1 (LIS1T-DNA Rice WD40 repeats Male fertility Plant height 

Notes

Acknowledgments

We thank Dr. Jian Xu for providing the plasmid pM999 and Dr. Gynheung An for providing the T-DNA insertional line 3A-04974. We thank Dr. Meizhong Luo for providing the Zhonghua 11 genomic DNA BAC clone a0023F18. This research was supported by grants from Program for New Century Excellent Talents in University, the National Natural Science Foundation of China, and the Key Project on Plant Transgenic Research.

Supplementary material

425_2011_1532_MOESM1_ESM.doc (1.6 mb)
Supplementary material 1 (DOC 1609 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Xinqiang Gao
    • 1
  • Zhihui Chen
    • 1
  • Jian Zhang
    • 1
  • Xingwang Li
    • 1
  • Guoxing Chen
    • 1
  • Xianghua Li
    • 1
  • Changyin Wu
    • 1
    Email author
  1. 1.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan)Huazhong Agricultural UniversityWuhanChina

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