Mutation of OsALDH7 causes a yellow-colored endosperm associated with accumulation of oryzamutaic acid A in rice
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Aldehyde dehydrogenase proteins consist of a superfamily and the family 7 (ALDH7) is a typical group with highly conserved proteins across species. It catalyzes oxidation of α-aminoadipic semialdehyde (AASA) in lysine degradation, participates in protection against hyperosmotic stress, and detoxifies aldehydes in human; however, its function in plants has been much less documented. Here we reported a mutant with yellow-colored endosperm in rice, and showed that the yellow endosperm was caused by mutation of OsALDH7. OsALDH7 is expressed in all tissues detected, with the highest level in mature seeds. We found that oryzamutaic acid A accumulated during late seed development and after a year-long storage in the colored endosperm, whereas it was undetectable in the wild type endosperm. Moreover, lysine degradation was enhanced in yeast over-expressing OsALDH7 and as a result, content of lysine, glutamate and saccharopine was changed, suggesting a role of OsALDH7 in lysine catabolism.
KeywordsAldehyde dehydrogenase 7 Yellow endosperm Oryzamutaic acid A Lysine catabolism Rice (Oryza sativa L.)
Days after flowering
Nicotinamide adenine dinucleotide
Nicotinamide adenine dinucleotide phosphate
This research was supported by the 973 Program of China (2007CB108802), the National Natural Science Foundation of China (30700497), National Key Transform Program (2008ZX08001-006), the Doctorial New Teacher Project from Education Department of China (20070307029), and the Commonweal Industry Scientific Research Program (200803056). We are grateful to Prof. Chuanying Wu (Ceres, Inc.) for critical reading of the manuscript.
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