, 234:903 | Cite as

Peroxisomal localisation of the final steps of the mevalonic acid pathway in planta

  • Andrew J. Simkin
  • Grégory Guirimand
  • Nicolas Papon
  • Vincent Courdavault
  • Insaf Thabet
  • Olivia Ginis
  • Sadok Bouzid
  • Nathalie Giglioli-Guivarc’h
  • Marc Clastre
Original Article


In plants, the mevalonic acid (MVA) pathway provides precursors for the formation of triterpenes, sesquiterpenes, phytosterols and primary metabolites important for cell integrity. Here, we have cloned the cDNA encoding enzymes catalysing the final three steps of the MVA pathway from Madagascar periwinkle (Catharanthus roseus), mevalonate kinase (MVK), 5-phosphomevalonate kinase (PMK) and mevalonate 5-diphosphate decarboxylase (MVD). These cDNA were shown to functionally complement MVA pathway deletion mutants in the yeast Saccharomyces cerevisiae. Transient transformations of C. roseus cells with yellow fluorescent protein (YFP)-fused constructs reveal that PMK and MVD are localised to the peroxisomes, while MVK was cytosolic. These compartmentalisation results were confirmed using the Arabidopsis thaliana MVK, PMK and MVD sequences fused to YFP. Based on these observations and the arguments raised here we conclude that the final steps of the plant MVA pathway are localised to the peroxisome.


Arabidopsis Catharanthus Isoprenoid Mevalonic acid pathway Peroxisome 



Acetoacetyl-CoA thiolase


5-fluoroorotic acid


3-hydroxy-3-methylglutaryl-CoA reductase


3-hydroxy-3-methylglutaryl-CoA synthase


Isopentenyl diphosphate isomerase


Mevalonic acid


Mevalonate 5-diphosphate decarboxylase


Mevalonate kinase


5-phosphomevalonate kinase


Peroxisomal targeting signal


Yellow fluorescent protein

Supplementary material

425_2011_1444_MOESM1_ESM.doc (234 kb)
Supplementary material 1 (DOC 234 kb)
425_2011_1444_MOESM2_ESM.doc (76 kb)
Supplementary material 2 (DOC 76 kb)
425_2011_1444_MOESM3_ESM.doc (72 kb)
Supplementary material 3 (DOC 71 kb)


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Andrew J. Simkin
    • 1
  • Grégory Guirimand
    • 1
  • Nicolas Papon
    • 1
  • Vincent Courdavault
    • 1
  • Insaf Thabet
    • 1
    • 2
  • Olivia Ginis
    • 1
  • Sadok Bouzid
    • 2
  • Nathalie Giglioli-Guivarc’h
    • 1
  • Marc Clastre
    • 1
  1. 1.EA 2106, Biomolécules et Biotechnologies VégétalesUniversité François-Rabelais de ToursToursFrance
  2. 2.Laboratoire de Biotechnologie et Physiologie Végétale, Département des Sciences BiologiquesFaculté des Sciences de TunisTunisTunisia

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