Planta

, Volume 234, Issue 3, pp 555–563 | Cite as

Environmental regulation of stomatal response in the Arabidopsis Cvi-0 ecotype

  • Keina Monda
  • Juntaro Negi
  • Atsuhiro Iio
  • Kensuke Kusumi
  • Mikiko Kojima
  • Mimi Hashimoto
  • Hitoshi Sakakibara
  • Koh Iba
Original Article

Abstract

The Arabidopsis Cape Verde Islands (Cvi-0) ecotype is known to differ from other ecotypes with respect to environmental stress responses. We analyzed the stomatal behavior of Cvi-0 plants, in response to environmental signals. We investigated the responses of stomatal conductance and aperture to high [CO2] in the Cvi-0 and Col-0 ecotypes. Cvi-0 showed constitutively higher stomatal conductance and more stomatal opening than Col-0. Cvi-0 stomata opened in response to light, but the response was slow. Under low humidity, stomatal opening was increased in Cvi-0 compared to Col-0. We then assessed whether low humidity affects endogenous ABA levels in Cvi-0. In response to low humidity, Cvi-0 had much higher ABA levels than Col-0. However, epidermal peels experiments showed that Cvi-0 stomata were insensitive to ABA. Measurements of organic and inorganic ions in Cvi-0 guard cell protoplasts indicated an over-accumulation of osmoregulatory anions (malate and Cl). This irregular anion homeostasis in the guard cells may explain the constitutive stomatal opening phenotypes of the Cvi-0 ecotype, which lacks high [CO2]-induced and low humidity-induced stomatal closure.

Keywords

Abscisic acid Arabidopsis Cape Verde Islands (Cvi-0) ecotype CO2 Stomata 

Abbreviations

ABA

Abscisic acid

FACE

Free air CO2 enrichment

FW

Fresh weight

GCP

Guard cell protoplast

gs

Stomatal conductance

JA

Jasmonic acid

O3

Ozone

RH

Relative humidity

SA

Salicylic acid

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Keina Monda
    • 1
  • Juntaro Negi
    • 1
  • Atsuhiro Iio
    • 2
  • Kensuke Kusumi
    • 1
  • Mikiko Kojima
    • 3
  • Mimi Hashimoto
    • 1
  • Hitoshi Sakakibara
    • 3
  • Koh Iba
    • 1
  1. 1.Department of Biology, Faculty of SciencesKyushu UniversityFukuokaJapan
  2. 2.National Institute for Environmental Studies (NIES)TsukubaJapan
  3. 3.RIKEN Plant Science CenterYokohamaJapan

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