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Planta

, Volume 234, Issue 3, pp 487–499 | Cite as

Ginseng root water-extracted pectic polysaccharides originate from secretory cavities

  • Li Yu
  • Yifa Zhou
  • J. Paul Knox
Original Article

Abstract

A range of molecular probes for cell wall polysaccharides has been used to explore the structure and location of water-extracted pectic polysaccharides occurring in fractions isolated from ginseng roots. The LM19 homogalacturonan (HG) epitope was abundant in an HG fraction and analysis of LM19 binding to a rhamnogalacturonan-I (RG-I) rich-fraction indicated that the LM19 epitope is sensitive to acetylation. A specific RG-I epitope (LM16), four arabinogalactan-protein (AGP) epitopes (LM2, LM14, JIM16, MAC207) and an extensin epitope (JIM20) were found to be abundant and co-located in several isolated polysaccharide fractions including an arabinogalactan fraction and two RG-I fractions. Detection of the RG-I, AGP and extensin epitopes identified in isolated polysaccharide fractions in sections of ginseng roots indicated that they were most abundant in secretory cavities found in the cortical regions of ginseng roots. In addition, the immunocytochemical study indicated that polysaccharide epitope masking is a widespread phenomenon in the primary cell walls of ginseng roots.

Keywords

Arabinogalactan-protein Cell walls Extensin Panax ginseng Homogalacturonan Secretory cavity 

Abbreviations

AGP

Arabinogalactan-protein

CBM

Carbohydrate-binding module

ELISA

Enzyme-linked immunosorbent assay

FITC

Fluorescein isothiocyanate

HG

Homogalacturonan

MP

Milk protein

PBS

Phosphate-buffered saline

PG

Endo-polygalacturonase

RG-I

Rhamnogalacturonan-I

TBO

Toluidine blue O

TFA

Trifluoroacetic acid

Notes

Acknowledgments

We are grateful to the Chinese Scholarship Council for the award of a travel scholarship to Li Yu. We thank Sue Marcus for technical assistance.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.School of Life SciencesNortheast Normal UniversityChangchunPeople’s Republic of China
  2. 2.Faculty of Biological Sciences, Centre for Plant SciencesUniversity of LeedsLeedsUK

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