Planta

, Volume 234, Issue 1, pp 195–205 | Cite as

Physiological consequences of desiccation in the aquatic bryophyte Fontinalis antipyretica

  • Ricardo Cruz de Carvalho
  • Cristina Branquinho
  • Jorge Marques da Silva
Original Article

Abstract

The moss Fontinalis antipyretica, an aquatic bryophyte previously described as desiccation-intolerant, is known to survive intermittent desiccation events in Mediterranean rivers. To better understand the mechanisms of desiccation tolerance in this species and to reconcile the apparently conflicting evidence between desiccation tolerance classifications and field observations, gross photosynthesis and chlorophyll a fluorescence were measured in field-desiccated bryophyte tips and in bryophyte tips subjected in the laboratory to slow, fast, and very fast drying followed by either a short (30 min) or prolonged (5 days) recovery. Our results show, for the first time, that the metabolic response of F. antipyretica to desiccation, both under field and laboratory conditions, is consistent with a desiccation-tolerance pattern; however, drying must proceed slowly for the bryophyte to regain its pre-desiccation state following rehydration. In addition, the extent of dehydration was found to influence metabolism whereas the drying rate determined the degree of recovery. Photosystem II (PSII) regulation and structural maintenance may be part of the induced desiccation tolerance mechanism allowing this moss to recover from slow drying. The decrease in the photochemical quenching coefficient (qP) immediately following rehydration may serve to alleviate the effects of excess energy on photosystem I (PSI), while low-level non-photochemical quenching (NPQ) would allow an energy shift enabling recovery subsequent to extended periods of desiccation. The findings were confirmed in field-desiccated samples, whose behavior was similar to that of samples slowly dried in the laboratory.

Keywords

Aquatic bryophytes Bryophyta Chlorophyll a fluorescence Desiccation tolerance Fontinalis Recovery 

Abbreviations

ANOVA

Analysis of variance

CO2

Carbon dioxide

DT

Desiccation tolerance

FDT

Full desiccation tolerance

Fv/Fm

Maximum quantum efficiency of photosystem II

MDT

Modified desiccation tolerance

NPQ

Non-photochemical quenching

O2

Oxygen

PAR

Photosynthetic active radiation

PSI

Photosystem I

PSII

Photosystem II

qP

Photochemical quenching coefficient

RH

Relative humidity

RWC

Relative water content

WC

Water content

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Ricardo Cruz de Carvalho
    • 1
  • Cristina Branquinho
    • 2
  • Jorge Marques da Silva
    • 1
  1. 1.Faculdade de Ciências (FC), Departamento de Biologia Vegetal (DBV) and Center for Biodiversity, Functional and Integrative Genomics (BioFIG)Universidade de Lisboa (UL)LisbonPortugal
  2. 2.Faculdade de Ciências (FC), Centro de Biologia Ambiental (CBA)Universidade de Lisboa (UL)LisbonPortugal

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