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Planta

, Volume 233, Issue 5, pp 1041–1053 | Cite as

Genomic organization, induced expression and promoter activity of a resistance gene analog (PmTNL1) in western white pine (Pinus monticola)

  • Jun-Jun Liu
  • Abul K. M. Ekramoddoullah
Original Article

Abstract

Cronartium ribicola causes white pine blister rust (WPBR) in subgenus Strobus. Various genetic and molecular approaches were used to detect white pine genes contributing to host resistance. The molecular role of the NBS-LRR family is highly related to plant immuno-activity against various pathogens and pests. In the present study, genomic organization of a resistance gene analog (RGA), designated as PmTNL1, and its allelic variants were characterized in Pinus monticola. PmTNL1 showed high identity with TIR-NBS-LRR proteins from other plants. qRT-PCR revealed that the PmTNL1 transcript was expressed at low basal levels in different tissues and exhibited similar patterns during compatible and incompatible interactions of P. monticola with C. ribicola at early stages post inoculation. In comparison, PmTNL1 was up-regulated significantly in diseased P. monticola tissues with WPBR symptoms. Expression of the PmTNL1 promoter::GUS fusion gene in transgenic Arabidopsis demonstrated that GUS signal appeared only inside phloem tissues of young seedlings and at hydathodes and branching and organ-connecting points in mature Arabidopsis plants. Similar to the endogenous expression pattern for this gene in pine, GUS activity was up-regulated significantly around vascular tissues locally at pathogen infection sites, but little or no induction was observed in response to abiotic stresses. A DNA marker was developed based on variation of the LRR-coding region, and PmTNL1 was mapped to one genetic linkage group using a pedigree with major dominant gene (Cr2) conferring HR resistance to C. ribicola. These results suggest that PmTNL1 may play an important role in white pine partial resistance against C. ribicola.

Keywords

Cronartium ribicola Genetic map Pinus Promoter activity analysis qRT-PCR TIR-NBS-LRR Transgenic Arabidopsis 

Abbreviations

GUS

β-Glucuronidase

HR

Hypersensitive reaction

I-PCR

Inverse-polymerase chain reaction

MGR

Major gene resistance

ORF

Open reading frame

qRT-PCR

Quantitative reverse transcription-polymerase chain reaction

RGA

Resistance gene analog

SNP

Single nucleotide polymorphism

TIR-NBS-LRR

Toll/Interleukin1 receptor-nucleotide binding site-leucine rich repeat

WPBR

White pine blister rust

WWP

Western white pine

Notes

Acknowledgments

This research was supported in part by the Canadian Forest Service and the CFS-Genomics R&D Initiative.

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Copyright information

© Her Majesty the Queen in Rights of Canada 2011

Authors and Affiliations

  1. 1.Pacific Forestry Centre, Canadian Forest ServiceNatural Resources CanadaVictoriaCanada

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