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Planta

, Volume 233, Issue 4, pp 763–772 | Cite as

Functional plant cell wall design revealed by the Raman imaging approach

  • Stephan Richter
  • Jörg Müssig
  • Notburga Gierlinger
Original Article

Abstract

Using the Raman imaging approach, the optimization of the plant cell wall design was investigated on the micron level within different tissue types at different positions of a Phormium tenax leaf. Pectin and lignin distribution were visualized and the cellulose microfibril angle (MFA) of the cell walls was determined. A detailed analysis of the Raman spectra extracted from the selected regions, allowed a semi-quantitative comparison of the chemical composition of the investigated tissue types on the micron level. The cell corners of the parenchyma revealed almost pure pectin and the cell wall an amount of 38–49% thereof. Slight lignification was observed in the parenchyma and collenchyma in the top of the leaf and a high variability (7–44%) in the sclerenchyma. In the cell corners and in the cell wall of the sclerenchymatic fibres surrounding the vascular tissue, the highest lignification was observed, which can act as a barrier and protection of the vascular tissue. In the sclerenchyma high variable MFA (4°–40°) was detected, which was related with lignin variability. In the primary cell walls a constant high MFA (57°–58°) was found together with pectin. The different plant cell wall designs on the tissue and microlevel involve changes in chemical composition as well as cellulose microfibril alignment and are discussed and related according to the development and function.

Keywords

Confocal Raman microscopy Lignin Microfibril orientation Pectin Plant cell wall Phormium 

Abbreviations

CC

Cell corners

Cle

Chlorenchyma

Col

Collenchyma

E

Epidermis

FCA

Fuchsin–chrysoidin–astrablue

MFA

Cellulose microfibril angle

P1 to P4

Position 1 to 4

Par

Parenchyma

Phl

Phloem

Sc

Sheath cells

Scl

Sclerenchyma

Sp

Spongy parenchyma

Vb

Vascular bundle

Xyl

Xylem

Notes

Acknowledgments

Notburga Gierlinger acknowledges financial support by the APART programme of the Austrian Academy of Sciences.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Stephan Richter
    • 1
  • Jörg Müssig
    • 1
  • Notburga Gierlinger
    • 2
    • 3
  1. 1.Faculty 5/Biomimetics, Biological MaterialsUniversity of Applied Sciences BremenBremenGermany
  2. 2.Department of BiomaterialsMax-Planck Institute of Colloids and InterfacesPotsdamGermany
  3. 3.Johannes Kepler University LinzInstitute of Polymer ScienceLinzAustria

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