Functional plant cell wall design revealed by the Raman imaging approach
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Using the Raman imaging approach, the optimization of the plant cell wall design was investigated on the micron level within different tissue types at different positions of a Phormium tenax leaf. Pectin and lignin distribution were visualized and the cellulose microfibril angle (MFA) of the cell walls was determined. A detailed analysis of the Raman spectra extracted from the selected regions, allowed a semi-quantitative comparison of the chemical composition of the investigated tissue types on the micron level. The cell corners of the parenchyma revealed almost pure pectin and the cell wall an amount of 38–49% thereof. Slight lignification was observed in the parenchyma and collenchyma in the top of the leaf and a high variability (7–44%) in the sclerenchyma. In the cell corners and in the cell wall of the sclerenchymatic fibres surrounding the vascular tissue, the highest lignification was observed, which can act as a barrier and protection of the vascular tissue. In the sclerenchyma high variable MFA (4°–40°) was detected, which was related with lignin variability. In the primary cell walls a constant high MFA (57°–58°) was found together with pectin. The different plant cell wall designs on the tissue and microlevel involve changes in chemical composition as well as cellulose microfibril alignment and are discussed and related according to the development and function.
KeywordsConfocal Raman microscopy Lignin Microfibril orientation Pectin Plant cell wall Phormium
Cellulose microfibril angle
- P1 to P4
Position 1 to 4
Notburga Gierlinger acknowledges financial support by the APART programme of the Austrian Academy of Sciences.
- Etzold H (2002) Simultanfärbung von Pflanzenschnitten mit Fuchsin, Chrysoidin und Astrablau. Mikrokosmos 91:316Google Scholar
- Gierlinger N, Schwanninger M (2007) The potential of Raman microscopy and Raman imaging in plant research. Spectrosc Int J 21:69–89Google Scholar
- Gindl W, Gupta HS, Schöberl T, Lichtenegger HC, Fratzl P (2004) Mechanical properties of spruce wood cell walls by nanoindentation. Appl Phys A Mater 79:2069–2073Google Scholar
- King MJ, Vincent JFV, Harris W (1996) Curling and folding of leaves of monocotyledons—a strategy for structural stiffness. N Z J Bot 34:411–416Google Scholar
- McIlroy RJ (1949) The hemicellulose of Phormium tenax (N. Z. flax). Part II. The constitution of the aldotrionic acid. J Chem Soc: 121–124Google Scholar
- McIlroy RJ, Holmes GS, Mauger RP (1945) A preliminary study of the polyuronide hemicellulose of Phormium tenax (N. Z. flax). J Chem Soc: 796–799Google Scholar
- Niklas KJ (1992) Plant biomechanics. An engineering approach to plant form and function. The University of Chicago Press, ChicagoGoogle Scholar
- Schmidt M, Schwartzberg AM, Perera PN, Weber-Bargioni A, Carroll A, Sarkar P, Bosneaga E, Urban JJ, Song J, Balakshin MY, Capanema EA, Auer M, Adams PD, Chiang VL, Schuck PJ (2009) Label-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa. Planta 230:589–597PubMedCrossRefGoogle Scholar