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Planta

, Volume 233, Issue 3, pp 485–494 | Cite as

Molecular analysis of an additional case of hybrid sterility in rice (Oryza sativa L.)

  • Z. G. Zhao
  • S. S. Zhu
  • Y. H. Zhang
  • X. F. Bian
  • Y. Wang
  • L. Jiang
  • X. Liu
  • L. M. Chen
  • S. J. Liu
  • W. W. Zhang
  • H. Ikehashi
  • J. M. WanEmail author
Original Article

Abstract

Hybrid sterility hinders the exploitation of the heterosis displayed by japonica × indica rice hybrids. The variation in pollen semi-sterility observed among hybrids between the japonica recipient cultivar and each of two sets of chromosome segment substitution lines involving introgression from an indica cultivar was due to a factor on chromosome 5 known to harbor the gene S24. S24 was fine mapped to a 42 kb segment by analyzing a large F2 population bred from the cross S24-NIL × Asominori, while the semi-sterility shown by the F1 hybrid was ascribable to mitotic failure at the early bicellular pollen stage. Interestingly, two other pollen sterility genes (f5-Du and Sb) map to the same region (Li et al. in Chin Sci Bull 51:675–680, 2006; Wang et al. in Theor Appl Genet 112:382–387, 2006), allowing a search for candidate genes in the 6.4 kb overlap between the three genes. By sequencing the overlapped fragment in wild rice, indica cultivars and japonica cultivars, a protein ankyrin-3 encoded by the ORF2 was identified as the molecular base for S24. A cultivar Dular was found to have a hybrid-sterility-neutral allele, S24-n, in which an insertion of 30 bp was confirmed. Thus, it was possible to add one more case of molecular bases for the hybrid sterility. No gamete abortion is caused on heterozygous maternal genotype with an impaired sequence from the hybrid-sterility-neutral genotype. This result will be useful in understanding of wide compatibility in rice breeding.

Keywords

Chromosome segment substitution lines Fine mapping Hybrid sterility Molecular breeding Proteins for gamete genesis 

Abbreviations

ANK-3

Ankyrin-3

CSSLs

Chromosome segment substitution lines

MMC

Microspore mother cell

NILs

Near isogenic lines

ORF

Open reading frame

SSR

Simple sequence repeat

WCG

Wide compatibility gene

WCV

Wide compatibility variety

Notes

Acknowledgments

We thank Dr. A. Yoshimura (Kyushu University, Japan) for his kind provision of the CSSL populations and associated genotypic data. This research was supported by the Grants from the Chinese National High Technology Research and Development Program (“863” Program, Nos. 2001AA241024; 2003AA222131; 2003AA207020), the Chinese National Natural Science Foundation (30800679) and the “948” project.

Supplementary material

425_2010_1313_MOESM1_ESM.doc (66 kb)
Supplementary material 1 (DOC 66 kb)
425_2010_1313_MOESM2_ESM.doc (50 kb)
Supplementary material 2 (DOC 49 kb)
425_2010_1313_MOESM3_ESM.doc (36 kb)
Supplementary material 3 (DOC 36 kb)
425_2010_1313_MOESM4_ESM.doc (33 kb)
Supplementary material 4 (DOC 33 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Z. G. Zhao
    • 1
  • S. S. Zhu
    • 2
  • Y. H. Zhang
    • 1
  • X. F. Bian
    • 1
  • Y. Wang
    • 1
  • L. Jiang
    • 1
  • X. Liu
    • 1
  • L. M. Chen
    • 1
  • S. J. Liu
    • 1
  • W. W. Zhang
    • 1
  • H. Ikehashi
    • 3
  • J. M. Wan
    • 1
    • 2
    Email author
  1. 1.National Key Laboratory For Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjingChina
  2. 2.Institute of Crop Science/The National Key Facility for Crop Gene Resources and Genetic ImprovementChinese Academy of Agricultural Sciences (CAAS)BeijingChina
  3. 3.Department of BreedingNihon UniversityFujisawaJapan

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