Planta

, Volume 233, Issue 3, pp 523–537

Comparative proteome analysis of metabolic changes by low phosphorus stress in two Brassica napus genotypes

  • Yinan Yao
  • Haiyan Sun
  • Fangsen Xu
  • Xuejiang Zhang
  • Shengyi Liu
Original Article

DOI: 10.1007/s00425-010-1311-x

Cite this article as:
Yao, Y., Sun, H., Xu, F. et al. Planta (2011) 233: 523. doi:10.1007/s00425-010-1311-x

Abstract

In an attempt to determine the adaptation strategy to phosphorous (Pi) deficiency in oilseed rape, comparative proteome analyses were conducted to investigate the differences of metabolic changes in two oilseed rape genotypes with different tolerance to low phosphorus (LP). Generally in either roots or leaves, there existed few low phosphorus (LP)-induced proteins shared in the two lines. The LP-tolerant genotype 102 maintained higher Pi concentrations than LP-sensitive genotype 105 when growing hydroponically under the 5-μM phosphorus condition. In 102 we observed the downregulation of the proteins related to gene transcription, protein translation, carbon metabolism, and energy transfer in leaves and roots, and the downregulation of proteins related to leaf growth and root cellular organization. But the proteins related to the formation of lateral root were upregulated, such as the auxin-responsive family proteins in roots and the sucrose-phosphate synthase-like protein in roots and leaves. On the other hand, the LP-sensitive genotype 105 maintained the low level of Pi concentrations and suffered high oxidative pressure under the LP condition, and stress-shocking proteins were pronouncedly upregulated such as the proteins for signal transduction, gene transcription, secondary metabolism, universal stress family proteins, as well as the proteins involved in lipid oxygenation and the disease resistance in both leaves and roots. Although the leaf proteins for growth in 105 were downregulated, the protein expressions in roots related to glycolysis and tricarboxylic acid (TCA) cycle were enhanced to satisfy the requirement of organic acid secretion.

Keywords

Comparative proteomics Brassica Low phosphorus tolerance 

Abbreviations

2-DE

Two-dimensional gel electrophoresis

APase

Acid phosphatase

GST

Glutathione transferase

HSPs

Heat shock proteins

IEF

Isoelectric focusing

IPG

Immobilized pH gradient

LP

Low phosphorus condition

MS

Flight mass spectrometry

P

Phosphorus

PMF

Peptide mass fingerprinting

ROS

Reactive oxygen species

SP

Sufficient phosphorus condition

TCA

Tricarboxylic acid

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Yinan Yao
    • 1
    • 2
  • Haiyan Sun
    • 1
  • Fangsen Xu
    • 3
  • Xuejiang Zhang
    • 1
  • Shengyi Liu
    • 1
  1. 1.Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research InstituteChinese Academy of Agricultural SciencesWuhanChina
  2. 2.Key Laboratory of Biogeography and Bioresources, Xinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqiChina
  3. 3.National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanChina

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