, Volume 233, Issue 2, pp 287–298 | Cite as

Advances in understanding pectin methylesterase inhibitor in kiwi fruit: an immunological approach

  • Evelien Vandevenne
  • Stefanie Christiaens
  • Sandy Van Buggenhout
  • Ruben P. Jolie
  • Margarita González-Vallinas
  • Thomas Duvetter
  • Paul J. Declerck
  • Marc E. Hendrickx
  • Ann Gils
  • Ann Van Loey
Original Article


In order to gain insight into the in situ properties and localisation of kiwi pectin methylesterase inhibitor (PMEI), a toolbox of monoclonal antibodies (MA) towards PMEI was developed. Out of a panel of MA generated towards kiwi PMEI, three MA, i.e. MA-KI9A8, MA-KI15C12 and MA-KI15G7, were selected. Thorough characterisation proved that these MA bind specifically to kiwi PMEI and kiwi PMEI in complex with plant PME and recognise a linear epitope on PMEI. Extract screening of green kiwi (Actinidia deliciosa) and gold kiwi (Actinidia chinensis) confirmed the potential use of these MA as probes to screen for PMEI in other sources. Tissue printing revealed the overall presence of PMEI in pericarp and columella of ripe kiwi fruit. Further analysis on the cellular level showed PMEI label concentrated in the middle lamella and in the cell-wall region near the plasmalemma. Intercellular spaces, however, were either completely filled or lined with label. In conclusion, the developed toolbox of antibodies towards PMEI can be used as probes to localise PMEI on different levels, which can be of relevance for plant physiologists as well as food technologists.


Immunolocalisation Kiwi fruit Monoclonal antibodies Pectin methylesterase inhibitor 



Aspergillus aculeatus pectin methylesterase


Monoclonal antibody


Pectin methylesterase


Pectin methylesterase inhibitor


Tomato (Solanum Lycopersicon) pectin methylesterase



This research has been carried out with financial support from the Research Council K.U.Leuven (IDO/05/011). S. Christiaens and S. Van Buggenhout are researchers funded by the Research Foundation - Flanders (FWO). The authors would like to acknowledge ProMeta, the Interfacultary Centre for Proteomics and Metabolomics for the MALDI-TOF-MS analysis. Sincere thanks goes to Prof. J. Hofkens for giving us access to Olympus Fluoview 1000 confocal microscope.


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Evelien Vandevenne
    • 1
  • Stefanie Christiaens
    • 1
  • Sandy Van Buggenhout
    • 1
  • Ruben P. Jolie
    • 1
  • Margarita González-Vallinas
    • 1
  • Thomas Duvetter
    • 1
  • Paul J. Declerck
    • 2
  • Marc E. Hendrickx
    • 1
  • Ann Gils
    • 2
  • Ann Van Loey
    • 1
  1. 1.Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre (LFoRCe), Department of Microbial and Molecular Systems (M2S)Katholieke Universiteit LeuvenLeuvenBelgium
  2. 2.Laboratory for Pharmaceutical Biology, Department of Pharmaceutical SciencesKatholieke Universiteit LeuvenLeuvenBelgium

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