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Planta

, Volume 233, Issue 1, pp 123–137 | Cite as

Molecular characterization of a miraculin-like gene differentially expressed during coffee development and coffee leaf miner infestation

  • Jorge Maurício Costa MondegoEmail author
  • Melina Pasini Duarte
  • Eduardo Kiyota
  • Leandro Martínez
  • Sandra Rodrigues de Camargo
  • Fernanda P. De Caroli
  • Beatriz Santos Capela Alves
  • Sandra Maria Carmello Guerreiro
  • Maria Luiza Vilela Oliva
  • Oliveiro Guerreiro-Filho
  • Marcelo Menossi
Original Article

Abstract

The characterization of a coffee gene encoding a protein similar to miraculin-like proteins, which are members of the plant Kunitz serine trypsin inhibitor (STI) family of proteinase inhibitors (PIs), is described. PIs are important proteins in plant defence against insects and in the regulation of proteolysis during plant development. This gene has high identity with the Richadella dulcifica taste-modifying protein miraculin and with the tomato protein LeMir; and was named as CoMir (Coffea miraculin). Structural protein modelling indicated that CoMir had structural similarities with the Kunitz STI proteins, but suggested specific folding structures. CoMir was up-regulated after coffee leaf miner (Leucoptera coffella) oviposition in resistant plants of a progeny derived from crosses between C. racemosa (resistant) and C. arabica (susceptible). Interestingly, this gene was down-regulated during coffee leaf miner herbivory in susceptible plants. CoMir expression was up-regulated after abscisic acid application and wounding stress and was prominent during the early stages of flower and fruit development. In situ hybridization revealed that CoMir transcripts accumulated in the anther tissues that display programmed cell death (tapetum, endothecium and stomium) and in the metaxylem vessels of the petals, stigma and leaves. In addition, the recombinant protein CoMir shows inhibitory activity against trypsin. According to the present results CoMir may act in proteolytic regulation during coffee development and in the defence against L. coffeella. The similarity of CoMir with other Kunitz STI proteins and the role of CoMir in plant development and plant stress are discussed.

Keywords

Coffee Kunitz STI proteinase inhibitor Miraculin- like protein Plant-insect interaction Xylem Programmed cell death 

Abbreviations

BAPA

Nα-Benzoyl-d, l-Arginin-p-nitroanilid

CV

Column volume

IMAC

Immobilized metal affinity chromatography

MLP

Miraculin-like protein

PI

Proteinase inhibitor

STI

Soybean trypsin inhibitor

RACE

Rapid amplification of cDNA end

Notes

Acknowledgments

The authors are grateful to Daniel Ramiro and Silvia Mathiessen (IAC) for rearing the insects, Dr. Nilson Ivo Zanchin (Laboratório Nacional de Luz Sincrotron) and Prof. Dr. Fabio Maranhão Costa (Departamento de Genética, Evolução e Bioagentes, IB, UNICAMP) for the use of the fluorescence microscopes, and Edna Santos (Departamento de Genética, Evolução e Bioagentes, IB, UNICAMP) and Sebastião Militão (Departamento de Biologia Vegetal, IB, UNICAMP) for technical assistance. J.M.C.M. and M.P.D. were recipients of fellowships from FAPESP (Fundação de Amparo a Pesquisa do Estado de São Paulo). L.M. received a PBIG-UNICAMP fellowship. S.C.R. received a fellowship from CAPES (Conselho de Aperfeiçoamento de Pesquisa em Ensino Superior). M.M. received a research fellowship from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico). This research was supported by the Consórcio Brasileiro de Pesquisa e Desenvolvimento do Café, FAEPEX/UNICAMP (project—040504), FAPESP (project—03/09361-4) and CNPq (479800/2004-9).

Supplementary material

425_2010_1284_MOESM1_ESM.doc (30 kb)
Supplementary material 1 (DOC 29 kb)
425_2010_1284_MOESM2_ESM.doc (27 kb)
Supplementary material 2 (DOC 27 kb)
425_2010_1284_MOESM3_ESM.doc (3.4 mb)
Supplementary material 3 (DOC 3493 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Jorge Maurício Costa Mondego
    • 1
    • 9
    Email author
  • Melina Pasini Duarte
    • 1
  • Eduardo Kiyota
    • 2
    • 3
  • Leandro Martínez
    • 4
  • Sandra Rodrigues de Camargo
    • 1
  • Fernanda P. De Caroli
    • 5
  • Beatriz Santos Capela Alves
    • 6
  • Sandra Maria Carmello Guerreiro
    • 7
  • Maria Luiza Vilela Oliva
    • 5
  • Oliveiro Guerreiro-Filho
    • 8
  • Marcelo Menossi
    • 1
  1. 1.Laboratório de Genoma Funcional Departamento de Genética, Evolução e Bioagentes, Instituto de BiologiaUniversidade Estadual de Campinas (UNICAMP)CampinasBrazil
  2. 2.Centro de Biologia Molecular e Engenharia GenéticaUniversidade Estadual de Campinas (UNICAMP)CampinasBrazil
  3. 3.Instituto de QuímicaUniversidade Estadual de Campinas (UNICAMP)CampinasBrazil
  4. 4.Instituto de Física de São CarlosUniversidade de São Paulo (USP), Grupo de CristalografiaSão CarlosBrazil
  5. 5.Departamento de BioquímicaUniversidade Federal de São Paulo (UNIFESP)São PauloBrazil
  6. 6.Laboratório Nacional de Biociências (LNBio)CampinasBrazil
  7. 7.Departamento de Biologia Vegetal, Instituto de BiologiaUniversidade Estadual de Campinas (UNICAMP)CampinasBrazil
  8. 8.Centro de Café Alcides CarvalhoInstituto Agronômico de Campinas (IAC)CampinasBrazil
  9. 9.Centro de Pesquisa e Desenvolvimento de Recursos Genéticos VegetaisInstituto Agronômico de Campinas (IAC)CampinasBrazil

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