Planta

, Volume 233, Issue 1, pp 49–61

Putative dual pathway of auxin transport in organogenesis of Arabidopsis

Original Article

DOI: 10.1007/s00425-010-1280-0

Cite this article as:
Banasiak, A. Planta (2011) 233: 49. doi:10.1007/s00425-010-1280-0

Abstract

In Arabidopsis, damage to the superficial acropetal polar auxin transport (PAT) inhibits generative but not vegetative organ initiation. In order to verify whether in a vegetative phase auxin can be transported to the meristem in a different way, the research on wild-type and plants with defective PAT was performed. Distance from the differentiated vascular elements to the shoot apical meristem (SAM) was measured for Arabidopsis cultured in different experimental systems. The influence of this distance on the ability to induce organogenesis as well as transport of the fluorescent dye to the SAM, and the LEAFY gene expression were analyzed. The youngest protoxylem elements were used as a marker of the vascular tissues. The distance of protoxylem to the SAM and organogenesis were interrelated. Organ initiation occurred only when protoxylem was localized near to the SAM. Experimental elongation of internodes in a vegetative rosette caused an increase in the distance between protoxylem and the SAM organogenic zone. Thus, the inhibition of organ initiation took place already during the vegetative phase. The results suggest the presence of at least two pathways of acropetal transport of auxin inducing organogenesis: one superficial way through PAT, and the second, putative one, internal through the vascular system. Possibly, organogenesis is completely blocked only when both these pathways are dysfunctional.

Keywords

Arabidopsis Organogenesis pin1 Polar auxin transport (PAT) Protoxylem Shoot apical meristem (SAM) 

Abbreviations

GA

Gibberellic acid

IAA

Indole-3-acetic acid

NPA

N-1-naphthylphthalamic acid

PAT

Polar auxin transport

SAM

Shoot apical meristem

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Institute of Plant BiologyUniversity of WrocławWrocławPoland

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