Planta

, Volume 232, Issue 6, pp 1325–1337

Expression and functional analysis of two genes encoding transcription factors, VpWRKY1 and VpWRKY2, isolated from Chinese wild Vitis pseudoreticulata

  • Huie Li
  • Yan Xu
  • Yu Xiao
  • Ziguo Zhu
  • Xiaoqing Xie
  • Heqing Zhao
  • Yuejin Wang
Original Article

Abstract

In this study, two WRKY genes were isolated from Erysiphe necator-resistant Chinese wild Vitis pseudoreticulata W. T. Wang ‘Baihe-35-1’, and designated as VpWRKY1 (GenBank accession no. GQ884198) and VpWRKY2 (GenBank accession no. GU565706). Nuclear localization of the two proteins was demonstrated in onion epidermal cells, while trans-activation function was confirmed in the leaves of ‘Baihe-35-1’. Expression of VpWRKY1 and VpWRKY2 was induced rapidly by salicylic acid treatment in ‘Baihe-35-1’. Expression of VpWRKY1 and VpWRKY2 was also induced rapidly by E. necator infection in 11 grapevine genotypes; the maximum induction of VpWRKY1 was greater in E. necator-resistant grapevine genotypes than in susceptible ones post E. necator inoculation. Furthermore, ectopic expression of VpWRKY1 or VpWRKY2 in Arabidopsis enhanced resistance to powdery mildew Erysiphe cichoracearum, and enhanced salt tolerance of transgenic plants. VpWRKY2 also enhanced cold tolerance of transgenic plants. In addition, the two proteins were shown to regulate the expression of some defense marker genes in Arabidopsis and grapevine. The data suggest that VpWRKY1 and VpWRKY2 may underlie the resistance in transgenic grapevine to E. necator and tolerance to salt and cold stresses.

Keywords

Chinese wild Vitis Disease resistance Grapevine Powdery mildew Transcription factor WRKY 

Abbreviations

qRT-PCR

Quantitative reverse transcriptase-polymerase chain reaction

VpWRKY1

Vitis pseudoreticulataWRKY1

VpWRKY2

Vitis pseudoreticulataWRKY2

SA

Salicylic acid

MeJA

Methyl jasmonate

Eth

Ethephon

Hpi

Hours post inoculation

dpi

Days post inoculation

hpt

Hours post treatment

EST

Expressed sequence tag

Supplementary material

425_2010_1258_MOESM1_ESM.doc (5.7 mb)
Supplementary material 1 (DOC 5850 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Huie Li
    • 1
    • 2
    • 3
  • Yan Xu
    • 1
    • 2
    • 3
  • Yu Xiao
    • 1
    • 2
    • 3
  • Ziguo Zhu
    • 1
    • 2
    • 3
  • Xiaoqing Xie
    • 1
    • 2
    • 3
  • Heqing Zhao
    • 1
    • 2
    • 3
  • Yuejin Wang
    • 1
    • 2
    • 3
  1. 1.College of HorticultureNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China, Ministry of Agriculture of the People’s Republic of ChinaNorthwest A&F UniversityYanglingPeople’s Republic of China
  3. 3.Shaanxi Key Laboratory of Molecular Biology of AgricultureNorthwest A&F UniversityYanglingPeople’s Republic of China

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