Planta

, Volume 232, Issue 3, pp 765–774

Expression of ethylene response factor JERF1 in rice improves tolerance to drought

  • Zhijin Zhang
  • Fang Li
  • Dingjun Li
  • Haiwen Zhang
  • Rongfeng Huang
Original Article

DOI: 10.1007/s00425-010-1208-8

Cite this article as:
Zhang, Z., Li, F., Li, D. et al. Planta (2010) 232: 765. doi:10.1007/s00425-010-1208-8

Abstract

Ethylene response factor (ERF) proteins regulate a variety of stress responses in plant. JERF1, a tomato ERF protein, can be induced by abscisic acid (ABA). Overexpression of JERF1 enhanced the tolerance of transgenic tobacco to high salt concentration, osmotic stress, and low temperature by regulating the expression of stress-responsive genes by binding to DRE/CRT and GCC-box cis-elements. In this research, we further report that overexpression of JERF1 significantly enhanced drought tolerance of transgenic rice. The overexpression activated the expression of stress-responsive genes and increased the synthesis of the osmolyte proline by regulating the expression of OsP5CS, encoding the proline biosynthesis key enzyme deltal-pyrroline-5-carboxylate synthetase. JERF1 also activated the expression of two ABA biosynthesis key enzyme genes, OsABA2 and Os03g0810800, and increased the synthesis of ABA in rice. Analysis of cis-elements of JERF1-targeted genes pointed to the existence of DRE/CRT and/or GCC box in their promoters, indicating that JERF1 could activate the expression of related genes in rice by binding to these cis-elements. Unlike some other ERF proteins, constructive overexpression of JERF1 did not change the growth and development of transgenic rice, which makes JEFR1 a potentially useful source in breeding for greater tolerance to abiotic stress.

Keywords

ERF protein Oryza sativa Drought Abscisic acid Transcriptional regulation 

Abbreviations

ABA

Abscisic acid

ERF

Ethylene response factor

WT

Wild-type rice

DRE/CRT

Dehydration-responsive element/C-repeat

h

Hour

Supplementary material

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Supplementary material 3 (JPEG 4,481 kb)
425_2010_1208_MOESM4_ESM.doc (34 kb)
Supplementary material 4 (DOC 34 kb)
425_2010_1208_MOESM5_ESM.doc (28 kb)
Supplementary material 5 (DOC 29 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Zhijin Zhang
    • 1
    • 3
    • 4
  • Fang Li
    • 1
    • 2
  • Dingjun Li
    • 2
  • Haiwen Zhang
    • 1
    • 3
    • 4
  • Rongfeng Huang
    • 1
    • 3
    • 4
  1. 1.Biotechnology Research InstituteChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Hunan Agricultural UniversityChangshaChina
  3. 3.National Key Facility of Crop Gene Resources and Genetic ImprovementBeijingChina
  4. 4.National Center for Plant Gene Research (Beijing)BeijingChina

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