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Planta

, Volume 232, Issue 3, pp 555–566 | Cite as

Arabidopsis DDB1a and DDB1b are critical for embryo development

  • Anne Bernhardt
  • Sutton Mooney
  • Hanjo Hellmann
Original Article

Abstract

DNA DAMAGED BINDING PROTEIN 1 (DDB1) is a highly conserved protein of around 125 kDa. It serves as a substrate adaptor subunit to a CUL4-based E3 ubiquitin ligase within the ubiquitin proteasome pathway. However, based on a set of three beta-propellers, the protein is able to mediate various protein–protein interactions, suggesting that it participates in many developmental and physiological processes in the plant. Arabidopsis encodes for two closely related DDB1 proteins, named DDB1a and DDB1b. While loss-of DDB1a does not severely affect development, loss-of DDB1b has been reported to result in an embryo lethal phenotype. Here we describe two novel ddb1b T-DNA insertion mutants that are not embryo lethal, which we utilized as genetic tools to dissect DDB1b from DDB1a function. Information generated by these studies showed that the C-terminal part of the DDB1 proteins is critical for specific protein–protein interactions. In addition, we demonstrated that DDB1a, like DDB1b, is critical for embryo development, and that both proteins have distinct functions in whole plant development.

Keywords

Arabidopsis DDB1 Embryo development Plant development 

Abbreviations

DDB1

DNA DAMAGED BINDING PROTEIN 1

DWD

DDB1-binding WD-40-box

ATCSA-1

COCKAYNE SYNDROME FACTOR A

PRL1

PLEIOTROPIC REGULATORY LOCUS 1

DET1

DE-ETIOLATED 1

COP1

CONSTITUTIVE PHOTOMORPHOGENIC 1

SPA1

SUPPRESSOR OF PHYA 1

MSI

MULTICOPY SUPPRESSOR OF IRA1

AG

AGAMOUS

AP3

APETALA3

Notes

Acknowledgments

We would like to thank the Deutsche Forschungsgemeinschaft (DFG grant HE3224/7-1 to H.H.) and Washington State University for supporting this project.

Supplementary material

425_2010_1195_MOESM1_ESM.pdf (109 kb)
Supplementary material 1 (PDF 108 kb)
425_2010_1195_MOESM2_ESM.pdf (241 kb)
Supplementary material 2 (PDF 240 kb)
425_2010_1195_MOESM3_ESM.pdf (370 kb)
Supplementary material 3 (PDF 369 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Anne Bernhardt
    • 1
    • 2
  • Sutton Mooney
    • 2
  • Hanjo Hellmann
    • 2
  1. 1.Freie UniversitätBerlinGermany
  2. 2.Washington State UniversityPullmanUSA

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