Planta

, Volume 232, Issue 2, pp 501–510

Production and identification of wheat-Agropyron cristatum 6P translocation lines

  • Yang Luan
  • Xiaoguang Wang
  • Weihua Liu
  • Chunye Li
  • Jinpeng Zhang
  • Ainong Gao
  • Yandong Wang
  • Xinming Yang
  • Lihui Li
Original Article

DOI: 10.1007/s00425-010-1187-9

Cite this article as:
Luan, Y., Wang, X., Liu, W. et al. Planta (2010) 232: 501. doi:10.1007/s00425-010-1187-9

Abstract

The narrow genetic background of wheat is the primary factor that has restricted the improvement of crop yield in recent years. The kernel number per spike is the most important factor of the many potential characteristics that determine wheat yield. Agropyron cristatum (L.) Gaertn., a wild relative of wheat, has the characteristics of superior numbers of florets and kernels per spike, which are controlled by chromosome 6P. In this study, the wheat-A. cristatum disomic addition and substitution lines were used as bridge materials to produce wheat-A. cristatum 6P translocation lines induced by gametocidal chromosomes and irradiation. The results of genomic in situ hybridization showed that the frequency of translocation induced by gametocidal chromosomes was 5.08%, which was higher than the frequency of irradiated hybrids (2.78%) and irradiated pollen (2.12%). The fluorescence in situ hybridization results of the translocation lines showed that A. cristatum chromosome 6P could be translocated to wheat ABD genome, and the recombination frequency was A genome > B genome > D genome. The alien A. cristatum chromosome 6P was translocated to wheat homoeologous groups 1, 2, 3, 5 and 6. We obtained abundant translocation lines that possessed whole-arm, terminal, segmental and intercalary translocations. Three 6PS-specific and four 6PL-specific markers will be useful to rapidly identify and trace the translocated fragments. The different wheat-A. cristatum 6P translocation lines obtained in this study can provide basic materials for analyzing the alien genes carried by chromosome 6P. The translocation line WAT33-1-3 and introgression lines WAI37-2 and WAI41-1, which had significant characteristics of multikernel (high numbers of kernels per spike), could be utilized as novel germplasms for high-yield wheat breeding.

Keywords

Wheat Agropyron cristatum Chromosome 6P Alien translocation line Multikernel 

Abbreviations

FISH

Fluorescence in situ hybridization

GISH

Genomic in situ hybridization

SSR

Simple sequence repeat

PCR

Polymerase chain reaction

SCAR

Sequence-characterized amplified region

STS

Sequence-tagged site

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Yang Luan
    • 1
  • Xiaoguang Wang
    • 1
  • Weihua Liu
    • 1
  • Chunye Li
    • 1
  • Jinpeng Zhang
    • 1
  • Ainong Gao
    • 1
  • Yandong Wang
    • 1
  • Xinming Yang
    • 1
  • Lihui Li
    • 1
  1. 1.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijingChina

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