, Volume 231, Issue 6, pp 1323–1334 | Cite as

BoPMEI1, a pollen-specific pectin methylesterase inhibitor, has an essential role in pollen tube growth

Original Article


Pectin methylesterase (PME) is known to have important roles in pollen development and pollen tube growth. As pivotal regulatory factors in PME activity modulation, PME inhibitors (PMEIs) are thought to be key regulators of cell wall stability at the tip of the pollen tube. We report on the cloning and characterization of a novel B. oleracea PMEI gene, BoPMEI1. Heterologously expressed BoPMEI1 showed PMEI activity. RT-PCR studies of different tissues and promoter-GUS fusions confirmed that BoPMEI1 was specifically expressed in mature pollen grains and pollen tubes. Based on in vivo transient assays, we found that BoPMEI1 appears to be largely localized to the plasma membrane. Transgenic Arabidopsis plants expressing antisense BoPMEI1 under the control of the CaMV 35S promoter suppressed the expression of the orthologous gene At1g10770, which led to partial male sterility and decreased seed set by inhibition of pollen tube growth. Our study demonstrates the involvement of BoPMEI1 in pollen tube growth.


Broccoli (B. oleracea var. italicaMale sterility Pectin methylesterase inhibitor Pollen tube growth 



Pectin methylesterase


Pectin methylesterase inhibitor


Reverse transcriptase polymerase chain reaction


35S Cauliflower mosaic virus 35S




cDNA-amplified fragment length polymorphism


Open reading frame


Kilometer Dalton




Amino acid


Isopropyl β-D-1-thiogalactopyranoside




Green-fluorescent protein


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Guo Yu Zhang
    • 1
  • Jing Feng
    • 1
  • Jian Wu
    • 1
  • Xiao Wu Wang
    • 1
  1. 1.Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China

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