Planta

, Volume 231, Issue 6, pp 1251–1260 | Cite as

AtCPK6, a functionally redundant and positive regulator involved in salt/drought stress tolerance in Arabidopsis

  • Jing Xu
  • Yong-Sheng Tian
  • Ri-He Peng
  • Ai-Sheng Xiong
  • Bo Zhu
  • Xiao-Fen Jin
  • Feng Gao
  • Xiao-Yan Fu
  • Xi-Lin Hou
  • Quan-Hong Yao
Original Article

Abstract

The calcium-dependent protein kinase (CDPK) family is needed in plant signaling during various physiological pathways. The Arabidopsis AtCPK6 gene belongs to the subclass of stress-inducible CDPKs, which is stimulated by salt and osmotic stress. To elucidate the physiological function of AtCPK6, transgenic Arabidopsis plants under the control of double CaMV 35S promoter were obtained. AtCPK6 over-expressing plants showed enhanced tolerance to salt/drought stresses. The elevated tolerance of the AtCPK6 over-expressing plants was confirmed by the change of proline and malondialdehyde (MDA). Real-time PCR analyses revealed that the expression levels of several stress-regulated genes were altered in AtCPK6 over-expressing plants. However, cpk6 mutant displayed no obvious difference with control. These results are likely to indicate that AtCPK6 is functionally redundant and a positive regulator involved in the tolerance to salt/drought stress in Arabidopsis.

Keywords

Arabidopsis Drought Functionally redundant Salt Stress tolerance 

Abbreviations

ABA

Abscisic acid

CDPK

Calcium-dependent protein kinase

MDA

Malondialdehyde

PEG

Polyethylene glycol

ROS

Reactive oxygen species

TBA

Thiobarbituric acid

Notes

Acknowledgments

We thank Prof. Julian I. Schroeder (University of California at San Diego, USA) for providing the seeds of loss-of-function mutant cpk6-1. This research was supported by Shanghai and National Natural Science Foundation (30670179, 08ZR1417200); 863 Program (2006AA10Z117, 2006AA06Z358, 2008AA10Z401); Shanghai Project for ISTC (08540706500); The Key Project Fund of the Shanghai Municipal Committee of Agriculture (No. 2008-7-5) and Shanghai Rising-Star Program (08QH14021).

Supplementary material

425_2010_1122_MOESM1_ESM.doc (33 kb)
Supplementary material 1 (DOC 33 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Jing Xu
    • 1
    • 2
  • Yong-Sheng Tian
    • 1
  • Ri-He Peng
    • 1
  • Ai-Sheng Xiong
    • 1
  • Bo Zhu
    • 1
  • Xiao-Fen Jin
    • 1
  • Feng Gao
    • 1
  • Xiao-Yan Fu
    • 1
  • Xi-Lin Hou
    • 2
  • Quan-Hong Yao
    • 1
  1. 1.Agro-Biotechnology Research InstituteShanghai Academy of Agricultural SciencesShanghaiChina
  2. 2.State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingChina

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