Planta

, Volume 231, Issue 4, pp 861–873 | Cite as

Cloning and characterization of the Gossypium hirsutum major latex protein gene and functional analysis in Arabidopsis thaliana

Original Article

Abstract

The major latex protein (MLP) gene in Gossypium hirsutum was cloned and designated Gh-MLP. Expression in cotton root was induced by salt stress and Verticillium dahliae toxin, and bioinformatic analysis showed that Gh-MLP encodes a 157-amino acid protein that is similar to members of the MLP subfamily in the Bet v 1 family. Although the structure of MLP is similar to Bet v 1 family proteins, the sequence identity to other subfamilies of Bet v 1 proteins is less than 20%. The Gh-MLP promoter contains potential cis-acting elements for response to salt stress and fungal elicitor. RT-PCR analysis showed that Gh-MLP expression was rapidly induced by NaCl and V. dahliae toxin, and induction was maintained over 72 h. However, Gh-MLP transgenic Arabidopsis thaliana did not show resistance to V. dahiae, salt tolerance was significantly enhanced. In contrast to the wild type, the Gh-MLP transgene allowed plants to germinate normally after treatment with 75 mM NaCl. Total flavonoid was twofold higher in transgenic Arabidopsis than in the control, suggesting that Gh-MLP might be involved in altering flavonoid content. We hypothesize Gh-MLP, like other Bet v 1 family proteins, participates in the binding or transport of ligands through its specific three-dimensional structure, and takes part in defensive responses to biotic and abiotic stresses.

Keywords

Flavonoid Induce expression Major latex proteins Salt stress Verticillium wilt resistance 

Abbreviations

CSBP

Cytokinin-specific binding proteins

min

Minute(s)

MLP

Major latex protein

NCS

(S)-norcoclaurine synthases

PR-10

Pathogenesis-related proteins family 10

RACE

Rapid amplification of cDNA ends

s

Second(s)

Notes

Acknowledgments

This work was funded by the National Natural Science Foundation of China (No. 30571210). We thank Rong-Qi Xu, Ting-Hui Zhou, Jia-Ni Wang, Feng-Xuan Zhao and Yu Weng for their technical assistance.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China

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