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Planta

, 231:349 | Cite as

Dynamic response of the transcriptome of a psychrophilic diatom, Chaetoceros neogracile, to high irradiance

  • Seunghye Park
  • Gyeongseo Jung
  • Yong-sic HwangEmail author
  • EonSeon JinEmail author
Original Article

Abstract

Large-scale RNA profiling revealed that high irradiance differentially regulated 577 out of 1,439 non-redundant genes of the Antarctic marine diatom Chaetoceros neogracile, represented on a custom cDNA chip, during 6 h of treatment. Among genes that were up- or down-regulated more than twofold within 30 min of treatment (310/1,439), about half displayed an acclimatory response during 6 h under high light. Expression of the remaining non-acclimatory genes also rapidly returned to initial levels within 30 min following a shift to low irradiance. High light altered expression of most of the photosynthesis genes (48/70), in contrast to genes in other functional categories. In addition, opposite response patterns were provoked in genes encoding fucoxanthin chlorophyll a/c binding protein (FCP), the main component of the diatom light-harvesting complex; high irradiance caused a decrease in expression of most FCP genes, but drove the rapid and specific up-regulation of ten others. C. neogracile responded very promptly to a change in light intensity by rapidly adjusting the transcript levels of FCP genes up-regulated by high light, and these dynamic adjustments coincided well with diatoxanthin (Dtx) levels formed by the xanthophyll cycle under the same conditions. The observation that the non-photochemical quenching (NPQ) capacity of this polar diatom was highly dependent on Dtx, which could bind to FCP and trigger NPQ, suggests that the up-regulated FCP gene products may participate in a photoprotective process as Dtx-binding proteins.

Keywords

Transcriptome Antarctic diatom High irradiance Xanthophyll cycle Fucoxanthin chlorophyll a/c binding protein NPQ (non-photochemical quenching) 

Abbreviations

DTT

Dithiothreitol

NPQ

Non-photochemical quenching

Ddx

Diadinoxanthin

Dtx

Diatoxanthin

DDE

Diadinoxanthin de-epoxidase

FCP

Fucoxanthin chlorophyll a/c binding protein

LHC

Light-harvesting complex

Notes

Acknowledgments

This work was supported by the Research Fund of Hanyang University (HY/2006/S).

Supplementary material

425_2009_1044_MOESM1_ESM.doc (1.5 mb)
Supplementary material 1 (DOC 1529 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Department of Life Science, College of Natural ScienceHanyang UniversitySeoulRepublic of Korea
  2. 2.Department of Bioscience and BiotechnologyKonkuk UniversitySeoulRepublic of Korea

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