Planta

, 230:517

Accumulation of hydroxycinnamic acid amides induced by pathogen infection and identification of agmatine coumaroyltransferase in Arabidopsis thaliana

  • Atsushi Muroi
  • Atsushi Ishihara
  • Chihiro Tanaka
  • Akihiro Ishizuka
  • Junji Takabayashi
  • Hideto Miyoshi
  • Takaaki Nishioka
Original Article

DOI: 10.1007/s00425-009-0960-0

Cite this article as:
Muroi, A., Ishihara, A., Tanaka, C. et al. Planta (2009) 230: 517. doi:10.1007/s00425-009-0960-0

Abstract

Hydroxycinnamic acid amides (HCAAs) are secondary metabolites involved in the defense of plants against pathogens. Here, we report the first identification of HCAAs, p-coumaroylagmatine, feruloylagmatine, p-coumaroylputrescine and feruloylputrescine, in Arabidopsis thaliana rosette leaves infected with Alternaria brassicicola and the assignment of At5g61160 as the agmatine coumaroyltransferase (AtACT) that catalyzes the last reaction in the biosynthesis of the HCAAs. Feeding experiments with putative labeled precursors revealed that the four HCAAs were synthesized from hydroxycinnamic acids and agmatine or putrescine. AtACT gene function was identified from an analysis of a mutant that did not accumulate HCAAs. In wild-type Arabidopsis, AtACT transcripts markedly increased in response to A. brassicicola infection. Enzymatic activity that catalyzes the synthesis of the HCAAs was confirmed in vitro by using a recombinant AtACT expressed in Escherichia coli. The Atact mutant was susceptible to infection by A. brassicicola, indicating that HCAAs are responsible for defense against pathogens in A.thaliana.

Keywords

Arabidopsis thaliana Alternaria brassicicola HCAA p-Coumaroylagmatine Agmatine coumaroyltransferase BAHD acyltransferase family 

Abbreviations

HCAA

Hydroxycinnamic acid amide

CouAgm

p-Coumaroylagmatine

FerAgm

Feruloylagmatine

CouPtr

p-Coumaroylputrescine

FerPtr

Feruloylputrescine

ACT

Agmatine coumaroyltransferase

AtACT

Arabidopsis thaliana agmatine coumaroyltransferase

Supplementary material

425_2009_960_MOESM1_ESM.doc (718 kb)
Supplementary material 1 (DOC 718 kb)

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Atsushi Muroi
    • 1
  • Atsushi Ishihara
    • 2
  • Chihiro Tanaka
    • 3
  • Akihiro Ishizuka
    • 4
  • Junji Takabayashi
    • 5
  • Hideto Miyoshi
    • 4
  • Takaaki Nishioka
    • 6
  1. 1.Division of Applied Biosciences, Graduate School of AgricultureKyoto UniversityKyotoJapan
  2. 2.Faculty of AgricultureTottori UniversityTottoriJapan
  3. 3.Division of Environmental Science and Technology, Graduate School of AgricultureKyoto UniversityKyotoJapan
  4. 4.Division of Applied Life Sciences, Graduate School of AgricultureKyoto UniversityKyotoJapan
  5. 5.Center for Ecological ResearchKyoto UniversityOtsuJapan
  6. 6.Institute for Advanced BiosciencesKeio UniversityTsuruokaJapan

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