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Planta

, Volume 230, Issue 1, pp 165–176 | Cite as

A novel insight into the regulation of light-independent chlorophyll biosynthesis in Larix decidua and Picea abies seedlings

  • Viktor DemkoEmail author
  • Andrej Pavlovič
  • Danka Valková
  • L’udmila Slováková
  • Bernhard Grimm
  • Ján Hudák
Original Article

Abstract

Light-independent chlorophyll (Chl) biosynthesis is a prerequisite for the assembly of photosynthetic pigment–protein complexes in the dark. Dark-grown Larix decidua Mill. seedlings synthesize Chl only in the early developmental stages and their Chl level rapidly declines during the subsequent development. Our analysis of the key regulatory steps in Chl biosynthesis revealed that etiolation of initially green dark-grown larch cotyledons is connected with decreasing content of glutamyl-tRNA reductase and reduced 5-aminolevulinic acid synthesizing capacity. The level of the Chl precursor protochlorophyllide also declined in the developing larch cotyledons. Although the genes chlL, chlN and chlB encoding subunits of the light-independent protochlorophyllide oxidoreductase were constitutively expressed in the larch seedlings, the accumulation of the ChlB subunit was developmentally regulated and ChlB content decreased in the fully developed cotyledons. The efficiency of chlB RNA-editing was also reduced in the mature dark-grown larch seedlings. In contrast to larch, dark-grown seedlings of Picea abies (L.) Karst. accumulate Chl throughout their whole development and show a different control of ChlB expression. Analysis of the plastid ultrastructure, photosynthetic proteins by Western blotting and photosynthetic parameters by gas exchange and Chl fluorescence measurements provide additional experimental proofs for differences between dark and light Chl biosynthesis in spruce and larch seedlings.

Keywords

Chlorophyll biosynthesis Light-independent protochlorophyllide oxidoreductase Gymnosperms Plastids RNA-editing 

Abbreviations

A

Net photosynthetic rate

ALA

5-Aminolevulinic acid

Chl

Chlorophyll

Chlide

Chlorophyllide

chlLNB

chlL, chlN, chlB genes

cpDNA

Plastid DNA

DPOR

Light-independent protochlorophyllide oxidoreductase

GluTR

Glutamyl-tRNA reductase

LHC2

Light-harvesting complexes associated with photosystem II

LPOR

Light-dependent NADPH-protochlorophyllide oxidoreductase

Pchlide

Protochlorophyllide

PLB

Prolamellar body

RD

Respiration rate

ФPSII

Effective quantum yield of PSII

Notes

Acknowledgments

This research work was supported by grants from the Slovak Research and Development Agency (APVV-20-020805), Scientific Grant Agency of the Ministry of Education of the Slovak Republic (VEGA 1/3288/06) given to J. H. and from the Sonderforschungsbereich (SFB 429-DFG) given to B. G. We thank Y. Fujita and J. Komenda for kindly providing the primary antibodies used in this work and Jarmila Šramková for excellent technical assistance.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Viktor Demko
    • 1
    • 3
    Email author
  • Andrej Pavlovič
    • 1
    • 3
  • Danka Valková
    • 2
  • L’udmila Slováková
    • 1
  • Bernhard Grimm
    • 3
  • Ján Hudák
    • 1
  1. 1.Department of Plant Physiology, Faculty of Natural SciencesComenius UniversityBratislavaSlovakia
  2. 2.Department of Molecular Biology, Faculty of Natural SciencesComenius UniversityBratislavaSlovakia
  3. 3.Institute of Biology/Plant PhysiologyHumboldt University BerlinBerlinGermany

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