, Volume 230, Issue 1, pp 13–25 | Cite as

Characterization of potato (Solanum tuberosum) and tomato (Solanum lycopersicum) protein phosphatases type 2A catalytic subunits and their involvement in stress responses

  • Silvia Marina País
  • Marina Alejandra González
  • María Teresa Téllez-Iñón
  • Daniela Andrea Capiati
Original Article


Protein phosphorylation/dephosphorylation plays critical roles in stress responses in plants. This report presents a comparative characterization of the serine/threonine PP2A catalytic subunit family in Solanum tuberosum (potato) and S. lycopersicum (tomato), two important food crops of the Solanaceae family, based on the sequence analysis and expression profiles in response to environmental stress. Sequence homology analysis revealed six isoforms in potato and five in tomato clustered into two subfamilies (I and II). The data presented in this work show that the expression of different PP2Ac genes is regulated in response to environmental stresses in potato and tomato plants and suggest that, in general, mainly members of the subfamily I are involved in stress responses in both species. However, the differences found in the expression profiles between potato and tomato suggest divergent roles of PP2A in the plant defense mechanisms against stress in these closely related species.


PP2A Solanum lycopersicum Solanum tuberosum Stress 



Protein phosphatase type 2A


Polygalacturonic acid


  1. Bai Y, Lindhout P (2007) Domestication and breeding of tomatoes: what have we gained and what can we gain in the future? Ann Bot (Lond) 100:1085–1094CrossRefGoogle Scholar
  2. Blakeslee JJ, Zhou HW, Heath JT, Skottke KR, Barrios JA, Liu SY, Delong A (2008) Specificity of RCN1-mediated protein phosphatase 2A regulation in meristem organization and stress response in roots. Plant Physiol 146:539–553PubMedCrossRefGoogle Scholar
  3. Bowles D (1998) Signal transduction in the wound response of tomato plants. Philos Trans R Soc Lond B Biol Sci 353:1495–1510PubMedCrossRefGoogle Scholar
  4. Büchter R, Strömberg A, Schmelzer E, Kombrink E (1997) Primary structure and expression of acidic (class II) chitinase in potato. Plant Mol Biol 35:749–761PubMedCrossRefGoogle Scholar
  5. Capiati DA, País SM, Téllez-Iñón MT (2006) Wounding increases salt tolerance in tomato plants: evidence on the participation of calmodulin-like activities in cross-tolerance signalling. J Exp Bot 57:2391–2400PubMedCrossRefGoogle Scholar
  6. Chandra S, Low PS (1995) Role of phosphorylation in elicitation of the oxidative burst in cultured soybean cells. Proc Natl Acad Sci USA 92:4120–4123PubMedCrossRefGoogle Scholar
  7. Cohen Y, Niderman T, Mösinger E, Fluhr R (1994) β-aminobutyric acid induces the accumulation of pathogenesis-related proteins in tomato (Lycorpesicon esculentum L.) plants and resistance to late blight infection caused by Phytophthora infestans. Plant Physiol 104:59–66PubMedGoogle Scholar
  8. De Wit PJ (2007) How plants recognize pathogens and defend themselves. Cell Mol Life Sci 64:2726–2732PubMedCrossRefGoogle Scholar
  9. Farkas I, Dombrádi V, Miskei M, Szabados L, Koncz C (2007) Arabidopsis PPP family of serine/threonine phosphatases. Trends Plant Sci 12:169–176PubMedCrossRefGoogle Scholar
  10. Farmer EE, Ryan CA (1990) Interplant communication: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proc Natl Acad Sci USA 87:7713–7718PubMedCrossRefGoogle Scholar
  11. Hassa P, Granado J, Freydl E, Wäspi U, Dudler R (2000) Syringolin-mediated activation of the Pir7b esterase gene in rice cells is suppressed by phosphatase inhibitors. Mol Plant Microbe Interact 13:342–346PubMedCrossRefGoogle Scholar
  12. He X, Anderson JC, del Pozo O, Gu YQ, Tang X, Martin GB (2004) Silencing of subfamily I of protein phosphatase 2A catalytic subunits results in activation of plant defense responses and localized cell death. Plant J 38:563–577PubMedCrossRefGoogle Scholar
  13. Hunter T (1995) Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling. Cell 80:225–236PubMedCrossRefGoogle Scholar
  14. Janssens V, Goris J (2001) Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling. Biochem J 353:417–439PubMedCrossRefGoogle Scholar
  15. Johnson R, Narvaez J, An G, Ryan C (1989) Expression of proteinase inhibitors I and II in transgenic tobacco plants: effects on natural defense against Manduca sexta larvae. Proc Natl Acad Sci USA 86:9871–9875PubMedCrossRefGoogle Scholar
  16. Kahn TL, Fender SE, Bray EA, O’Connell MA (1993) Characterization of expression of drought- and abscisic acid-regulated tomato genes in the drought-resistant species Lycopersicon pennellii. Plant Physiol 103:597–605PubMedGoogle Scholar
  17. Lecourieux-Ouaked F, Pugin A, Lebrun-Garcia A (2000) Phosphoproteins involved in the signal transduction of cryptogein, an elicitor of defense reactions in tobacco. Mol Plant Microbe Interact 13:821–829PubMedCrossRefGoogle Scholar
  18. Loke JC, Stahlberg EA, Strenski DG, Haas BJ, Wood PC, Li QQ (2005) Compilation of mRNA polyadenylation signals in Arabidopsis revealed a new signal element and potential secondary structures. Plant Physiol 138:1457–1468PubMedCrossRefGoogle Scholar
  19. Longin S, Zwaenepoel K, Louis JV, Dilworth S, Goris J, Janssens V (2007) Selection of protein phosphatase 2A regulatory subunits is mediated by the C-terminus of the catalytic subunit. J Biol Chem 282:26971–26980PubMedCrossRefGoogle Scholar
  20. Luan S (2003) Protein phosphatases in plants. Annu Rev Plant Biol 54:63–92PubMedCrossRefGoogle Scholar
  21. Orozco-Cardenas M, McGurl B, Ryan CA (1993) Expression of an antisense prosystemin gene in tomato plants reduces resistance toward Manduca sexta larvae. Proc Natl Acad Sci USA 90:8273–8276PubMedCrossRefGoogle Scholar
  22. Prickett TD, Brautigan DL (2006) The alpha4 regulatory subunit exerts opposing allosteric effects on protein phosphatases PP6 and PP2A. J Biol Chem 281:30503–30511PubMedCrossRefGoogle Scholar
  23. Rensink WA, Lobst S, Hart A, Stegalkina S, Liu J, Buell CR (2005) Gene expression profiling of potato responses to cold, heat, and salt stress. Funct Integr Genomics 5:201–207PubMedCrossRefGoogle Scholar
  24. Schönthal AH (1998) Role of PP2A in intracellular signal transduction pathways. Front Biosci 3:D1262–D1273PubMedGoogle Scholar
  25. Shen Y, Ji G, Haas BJ, Wu X, Zheng J, Reese GJ, Li QQ (2008) Genome level analysis of rice mRNA 3′-end processing signals and alternative polyadenylation. Nucleic Acids Res 36:3150–3161PubMedCrossRefGoogle Scholar
  26. Spooner DM, McLean K, Ramsay G, Waugh R, Bryan GJ (2007) A single domestication for potato based on multilocus amplified fragment length polymorphism genotyping. Proc Natl Acad Sci USA 102:14694–14990CrossRefGoogle Scholar
  27. Tóth EC, Vissi E, Kovács I, Szöke A, Ariño J, Gergely P, Dudits D, Dombrádi V (2000) Protein phosphatase 2A holoenzyme and its subunits from Medicago sativa. Plant Mol Biol 43:527–536PubMedCrossRefGoogle Scholar
  28. Virshup DM (2000) Protein phosphatase 2A: a panoply of enzymes. Curr Opin Cell Biol 12:180–185PubMedCrossRefGoogle Scholar
  29. Wei H, Ashby DG, Moreno CS, Ogris E, Yeong FM, Corbett AH, Pallas DC (2001) Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B-type subunits Cdc55p and Rts1p. J Biol Chem 276:1570–1577PubMedCrossRefGoogle Scholar
  30. Xu Y, Xing Y, Chen Y, Chao Y, Lin Z, Fan E, Yu JW, Strack S, Jeffrey PD, Shi Y (2006) Structure of protein phosphatase 2A holoenzyme. Cell 127:1239–1251PubMedCrossRefGoogle Scholar
  31. Xu C, Jing R, Mao X, Jia X, Chang X (2007) A wheat (Triticum aestivum) protein phosphatase 2A catalytic subunit gene provides enhanced drought tolerance in tobacco. Ann Bot (Lond) 99:439–450CrossRefGoogle Scholar
  32. Yamaguchi-Shinozaki K, Shinozaki K (2005) Organization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters. Trends Plant Sci 10:88–94PubMedCrossRefGoogle Scholar
  33. Yu XX, Du X, Moreno CS, Green RE, Ogris E, Feng Q, Chou L, McQuoid MJ, Pallas DC (2001) Methylation of the protein phosphatase 2A catalytic subunit is essential for association of B-alpha regulatory subunit but not SG2NA, striatin, or polyomavirus middle tumor antigen. Mol Biol Cell 12:185–199PubMedGoogle Scholar
  34. Yu RM, Zhou Y, Xu ZF, Chye ML, Kong RY (2003) Two genes encoding protein phosphatase 2A catalytic subunits are differentially expressed in rice. Plant Mol Biol 51:295–311PubMedGoogle Scholar
  35. Zhang Z, Zhao S, Long F, Zhang L, Bai G, Shima H, Nagao M, Lee EYC (1994) A mutant of protein phosphatase-1 that exhibits altered toxin sensitivity. J Biol Chem 269:16997–17000PubMedGoogle Scholar
  36. Zhang X, Fowler SG, Cheng H, Lou Y, Rhee SY, Stockinger EJ, Thomashow MF (2004) Freezing-sensitive tomato has a functional CBF cold response pathway, but a CBF regulon that differs from that of freezing-tolerant Arabidopsis. Plant J 39:905–919PubMedCrossRefGoogle Scholar
  37. Zhuo S, Clemens JC, Stone RL, Dixon JE (1994) Mutational analysis of a Ser/Thr phosphatase. Identification of residues important in phosphoesterase substrate binding and catalysis. J Biol Chem 269:26234–26238PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Silvia Marina País
    • 1
  • Marina Alejandra González
    • 1
  • María Teresa Téllez-Iñón
    • 1
  • Daniela Andrea Capiati
    • 1
  1. 1.Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresCiudad de Buenos AiresArgentina

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