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Planta

, Volume 229, Issue 4, pp 955–964 | Cite as

Overexpression of AtHsp90.2, AtHsp90.5 and AtHsp90.7 in Arabidopsis thaliana enhances plant sensitivity to salt and drought stresses

  • Hongmiao Song
  • Rongmin ZhaoEmail author
  • Pengxiang Fan
  • Xuchu Wang
  • Xianyang Chen
  • Yinxin LiEmail author
Original Article

Abstract

Three AtHsp90 isoforms, cytosolic AtHsp90.2, chloroplast-located AtHsp90.5, and endoplasmic reticulum (ER)-located AtHsp90.7, were characterized by constitutive overexpressing their genes in Arabidopsis thaliana. Both types of the transgenic plants overexpressing cytosolic and organellar AtHsp90s showed reduced tolerance to salt and drought stresses with lower germination rates and fresh weights, but improved tolerance to high concentration of Ca2+ comparing with the wild type plants. Transcriptional analysis of ABA-responsive genes, RD29A, RD22 and KIN2 under salt and drought stresses, indicated that the induction expression of these genes was delayed by constitutive overexpression of cytosolic AtHsp90.2, but was hardly affected by that of organellar AtHsp90.5 and AtHsp90.7. These results implied that Arabidopsis different cellular compartments-located Hsp90s in Arabidopsis might be involved in abiotic stresses by different functional mechanisms, probably through ABA-dependent or Ca2+ pathways, and proper homeostasis of Hsp90 was critical for cellular stress response and/or tolerance in plants.

Keywords

Arabidopsis Calcium tolerance Heat shock protein 90 Stress sensitivity Signaling pathway 

Abbreviations

ABA

Abscisic acid

CaMV

Cauliflower mosaic virus

Hsc

Cognate form of Hsp

Hsp

Heat shock protein

MAPK

Mitogen-activated protein kinase

ROS

Reactive oxygen species

UPR

Unfolded protein response

Notes

Acknowledgments

We thank Dr. Walid Houry (University of Toronto, Canada) for providing us with polyclonal antibody against yeast Hsp82 protein. This work is supported by the National Natural Science Foundation of China (Grant No.30470352) and the National High Technology and Research Development Program of China (“863” project, Grant No. 2007AA091705).

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Key Laboratory of Photosynthesis and Environmental Molecular PhysiologyInstitute of Botany, Chinese Academy of SciencesBeijingChina
  2. 2.Department of Biological SciencesUniversity of Toronto ScarboroughTorontoCanada

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