Planta

, Volume 229, Issue 2, pp 249–259 | Cite as

The promoter of the pepper pathogen-induced membrane protein gene CaPIMP1 mediates environmental stress responses in plants

Original Article
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Abstract

The promoter of the pepper pathogen-induced membrane protein gene CaPIMP1 was analyzed by an Agrobacterium-mediated transient expression assay in tobacco leaves. Several stress-related cis-acting elements (GT-1, W-box and ABRE) are located within the CaPIMP1 promoter. In tobacco leaf tissues transiently transformed with a CaPIMP1 promoter-β-glucuronidase (GUS) gene fusion, serially 5′-deleted CaPIMP1 promoters were differentially activated by Pseudomonas syringae pv. tabaci, ethylene, methyl jasmonate, abscisic acid, and nitric oxide. The −1,193 bp region of the CaPIMP1 gene promoter sequence exhibited full promoter activity. The −417- and −593 bp promoter regions were sufficient for GUS gene activation by ethylene and methyl jasmonate treatments, respectively. However, CaPIMP1 promoter sequences longer than −793 bp were required for promoter activation by abscisic acid and sodium nitroprusside treatments. CaPIMP1 expression was activated in pepper leaves by treatment with ethylene, methyl jasmonate, abscisic acid, β-amino-n-butyric acid, NaCl, mechanical wounding, and low temperature, but not with salicylic acid. Overexpression of CaPIMP1 in Arabidopsis conferred hypersensitivity to mannitol, NaCl, and ABA during seed germination but not during seedling development. In contrast, transgenic plants overexpressing CaPIMP1 exhibited enhanced tolerance to oxidative stress induced by methyl viologen during germination and early seedling stages. These results suggest that CaPIMP1 expression may alter responsiveness to environmental stress, as well as to pathogen infection.

Keywords

Capsicum annuum Osmotic stress Oxidative damage Plasma membrane protein Promoter activation Transgenic Arabidopsis 

Abbreviations

ABA

Abscisic acid

BABA

β-Amino-n-butyric acid

ERE

Ethylene-responsive element

GUS

β-Glucuronidase

MeJA

Methyl jasmonate

MV

Methyl viologen

OX

Overexpression

PR

Pathogenesis-related

SA

Salicylic acid

SNP

Sodium nitroprusside

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Notes

Acknowledgments

This research was supported by a grant (CG1133) from the Crop Functional Genomics Center of the 21st Century, Frontier Research Program, funded by the Ministry of Science and Technology, Korea.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of Horticulture, College of Life Sciences and Natural ResourcesJinju National UniversityJinjuRepublic of Korea
  2. 2.Laboratory of Molecular Plant Pathology, School of Life Sciences and BiotechnologyKorea UniversitySeoulRepublic of Korea

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