, 228:929 | Cite as

Two FLOWERING LOCUS T (FT) homologs in Chenopodium rubrum differ in expression patterns

  • David Cháb
  • Jan Kolář
  • Matthew S. Olson
  • Helena Štorchová
Original Article


FLOWERING LOCUS T (FT) like genes are crucial regulators (both positive and negative) of flowering in angiosperms. We identified two FT homologs in Chenopodium rubrum, a short-day species used as a model plant for the studies of photoperiodic flower induction. We found that CrFTL1 gene was highly inducible by a 12-h dark period, which in turn induced flowering. On the other hand, photoperiodic treatments that did not induce flowering (short dark periods, or a permissive darkness interrupted by a night break) caused only a slight increase in CrFTL1 mRNA level. We demonstrated diurnal oscillation of CrFTL1 expression with peaks in the middle of a light period. The oscillation persisted under constant darkness. Unlike FT homologs in rice and Pharbitis, the CrFTL1 expression under constant darkness was very low. The CrFTL2 gene showed constitutive expression. We suggest that the CrFTL1 gene may play a role as a floral regulator, but the function of CrFTL2 remains unknown.


Chenopodium FLOWERING LOCUS T homologs Flower induction Gene expression Night break 





Arabidopsis thaliana CENTRORADIALIS homolog












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  1. Ahn JH, Miller D, Winter VJ, Banfield MJ, Lee JH, Yoo SY, Henz SR, Brady RL, Weigel D (2006) A divergent external loop confers antagonistic activity on floral regulators FT and TFL1. EMBO J 25:605–614PubMedCrossRefGoogle Scholar
  2. Blažková A, Vondráková Z, Krekule J (2000) The shoot apex as a marker of the responsivity to photoperiodic treatment inducing flowering of Chenopodium rubrum L. Biol Plant 43:31–34CrossRefGoogle Scholar
  3. Blazquez MA, Ahn JH, Weigel D (2003) A thermosensory pathway controlling flowering time in Arabidopsis thaliana. Nat Genet 33:168–171PubMedCrossRefGoogle Scholar
  4. Bradley D, Ratcliffe O, Vincent C, Carpenter R, Coen C (1997) Inflorescence commitment and architecture in Arabidopsis. Science 275:80–83PubMedCrossRefGoogle Scholar
  5. Carmel-Goren L, Liu YS, Lifschitz E, Zamir D (2003) The SELF-PRUNING gene family in tomato. Plant Mol Biol 52:1215–1222PubMedCrossRefGoogle Scholar
  6. Cumming BG (1967) Early-flowering plants. In: Wilt FH, Wessels NK (eds) Methods in developmental biology. Crowell, New York, pp 277–299Google Scholar
  7. Cumming BG, Seabrook JEA (1985) Chenopodium. In: Halevy AH (ed) CRC handbook of flowering, vol II. CRC Press, Boca Raton, pp 96–228Google Scholar
  8. Cumming BG, Hendricks SB, Borthwick HA (1965) Rhythmic flowering responses and phytochrome changes in a selection of Chenopodium rubrum. Can J Bot 43:825–853CrossRefGoogle Scholar
  9. Doi K, Izawa T, Fuse T, Yamaguchi U, Kubo T, Shimatani Z, Yano M, Yoshimura A (2004) Ehd1, a B-type response regulator in rice, confers short-day promotion of flowering and controls FT like gene expression independently of Hd1. Gene Dev 18:926–936PubMedCrossRefGoogle Scholar
  10. Felsenstein J (2004) Inferring phylogenies. Sinauer Associates, Inc, Sunderland MassGoogle Scholar
  11. Hayama R, Yokoi S, Tamaki S, Yano M, Shimamoto K (2003) Adaptation of photoperiodic control pathways produces short-day flowering in rice. Nature 422:719–722PubMedCrossRefGoogle Scholar
  12. Hayama R, Agashe B, Luley E, King R, Coupland G (2007) A circadian rhythm set by dusk determines the expression of FT homologs and the short-day photoperiodic flowering response in Pharbitis. Plant Cell 19:2988–3000PubMedCrossRefGoogle Scholar
  13. Hecht V, Foucher F, Ferrandiz C, Macknight R, Navarro C, Morin J, Vardy ME, Ellis N, Beltran JP, Rameau C, Weller JL (2005) Conservation of Arabidopsis flowering genes in model legumes. Plant Physiol 137:1420–1434PubMedCrossRefGoogle Scholar
  14. Hsu CY, Liu YX, Luthe DS, Yuceer C (2006) Poplar FT2 shortens the juvenile phase and promotes seasonal flowering. Plant Cell 18:1846–1861PubMedCrossRefGoogle Scholar
  15. Huang JC, Chen F (2006) Simultaneous amplification of 5′ and 3′ cDNA ends based on template-switching effect and inverse PCR. BioTechniques 40:187–189PubMedCrossRefGoogle Scholar
  16. Ishikawa R, Tamaki S, Yokoi S, Inagaki N, Shinomura T, Takano M, Shimamoto K (2005) Suppression of the floral activator Hd3a is the principal cause of the night break effect in rice. Plant Cell 17:3326–3336PubMedCrossRefGoogle Scholar
  17. Jaeger KE, Wigge PA (2007) FT protein acts as a long-range signal in Arabidopsis. Curr Biol 17:1050–1054PubMedCrossRefGoogle Scholar
  18. Kardailsky I, Shukla V, Ahn JH, Dagenais N, Christensen SK, Nguyen JT, Chory J, Harrison MJ, Weigel D (1999) Activation tagging of the floral inducer FT. Science 286:1962–1965PubMedCrossRefGoogle Scholar
  19. King RW (1972) Timing in Chenopodium rubrum of export of the floral stimulus from the cotyledons and its action at the shoot apex. Can J Bot 50:697–702CrossRefGoogle Scholar
  20. Kobayashi Y, Kaya H, Goto K, Iwabuchi M, Araki T (1999) A pair of related genes with antagonistic roles in mediating flowering signals. Science 286:1960–1962PubMedCrossRefGoogle Scholar
  21. Kojima S, Takahashi Y, Kobayashi Y, Monna L, Sasaki T, Araki T, Yano M (2002) Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short day conditions. Plant Cell Physiol 43:1096–1105PubMedCrossRefGoogle Scholar
  22. Lee JH, Hong SM, Yoo SJ, Park OK, Lee JS, Ahn JH (2006) Integration of floral inductive signals by flowering locus T and supressor of overexpression of Constans 1. Physiol Plantarum 126:475–483Google Scholar
  23. Lynch M, Force A (2000) The probability of duplicate gene preservation by subfunctionalization. Genetics 154:459–473PubMedGoogle Scholar
  24. Mimida N, Goto K, Kobayashi Y, Araki T, Ahn JH, Weigel D, Murata M, Motoyoshi F, Sakamoto W (2001) Functional divergence of the TFL1-like gene family in Arabidopsis revealed by characterization of a novel homologue. Genes Cells 6:327–336PubMedCrossRefGoogle Scholar
  25. Ohno S (1970) Evolution by gene duplication. Springer-Verlag, BerlinGoogle Scholar
  26. Otto SP, Yong P (2002) The evolution of gene duplicates. Adv Genet 46:451–483PubMedCrossRefGoogle Scholar
  27. Partap T, Joshi BD, Galway NW (1998) Chenopods. Chenopodium spp. Promoting the conservation and use of underutilized and neglected crops, vol 22. Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute, RomeGoogle Scholar
  28. Putterill J, Robson F, Lee K, Simon R, Coupland G (1995) The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors. Cell 80:847–857PubMedCrossRefGoogle Scholar
  29. Rahiminejad MR, Gornall RJ (2004) Flavonoid evidence for allopolyploidy in the Chenopodium album aggregate (Amaranthaceae). Plant Syst Evol 246:77–87CrossRefGoogle Scholar
  30. Samach A, Onouchi H, Gold SE, Ditta GS, Schwarz-Sommer Z, Yanofsky MF, Coupland G (2000) Distinct roles of CONSTANS target genes in reproductive development of Arabidopsis. Science 288:1613–1616PubMedCrossRefGoogle Scholar
  31. Schoentgen F, Saccoccio F, Jolles J, Bernier I, Jolles P (1987) Complete amino-acid-sequence of a basic 21 kDa protein from bovine brain cytosol. Euro J Biochem 166:333–338CrossRefGoogle Scholar
  32. Seidlová F, Krekule J (1973) The negative response of photoperiodic floral induction in Chenopodium rubrum L. to preceding growth. Ann Bot 37:605–614Google Scholar
  33. Štorchová H, Hrdličková R, Chrtek J Jr, Tetera M, Fitze D, Fehrer J (2000) An improved method of DNA isolation from plants collected in the field and conserved in saturated NaCl/CTAB solution. Taxon 49:79–84CrossRefGoogle Scholar
  34. Suarez-Lopez P, Wheatley K, Robson F, Onouchi H, Valverde F, Coupland G (2001) CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis. Nature 410:1116–1120PubMedCrossRefGoogle Scholar
  35. Swofford DL (2003) PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, SunderlandGoogle Scholar
  36. Tamaki S, Matsuo S, Wong HL, Yokoi S, Shimamoto K (2007) Hd3a protein is a mobile flowering signal in rice. Science 316:1033–1036PubMedCrossRefGoogle Scholar
  37. Ullmann J, Seidlová F, Krekule J, Pavlová L (1985) Chenopodium rubrum as a model plant for testing the flowering effects of PGRs. Biol Plant 27:367–372CrossRefGoogle Scholar
  38. Veit J, Wagner E, Albrechtová JTP (2004) Isolation of a FLORICAULA/LEAFY putative orthologue from Chenopodium rubrum and its expression during photoperiodic flower induction. Plant Physiol Biochem 42:573–578PubMedCrossRefGoogle Scholar
  39. Wada M, Cao Q, Nobuhiro K, Soejima J, Masuda T (2002) Apple has two orthologs of FLORICAULA/LEAFY involved in flowering. Plant Mol Biol 49:567–577PubMedCrossRefGoogle Scholar
  40. Wigge PA, Kim MC, Jaeger KE, Busch W, Schmid M, Lohman JU, Weigel D (2005) Integration of spatial and temporal information during floral induction in Arabidopsis. Science 309:1056–1059PubMedCrossRefGoogle Scholar
  41. Yamaguchi A, Kobayashi Y, Goto K, Abe M, Araki T (2005) TWIN SISTER OF FT (TSF) acts as a floral pathway integrator redundantly with FT. Plant Cell Physiol 46:1175–1189PubMedCrossRefGoogle Scholar
  42. Yano M, Katayose Y, Ashikari M, Yamanouchi U, Monna L, Fuse T, Baba T, Yamamoto K, Umehara Y, Nagamura Y, Sasaki T (2000) Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell 12:2473–2484PubMedCrossRefGoogle Scholar
  43. Yoo SY, Kardailsky I, Lee JS, Weigel D, Ahn JH (2004) Acceleration of flowering by overexpression of MFT (MOTHER OF FT AND TFL1). Mol Cells 17:95–101PubMedGoogle Scholar
  44. Zeevaart JAD (1976) Physiology of flower formation. Annu Rev Plant Physiol Plant Mol Biol 27:321–348Google Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • David Cháb
    • 1
  • Jan Kolář
    • 1
  • Matthew S. Olson
    • 2
    • 3
  • Helena Štorchová
    • 1
    • 3
  1. 1.Institute of Experimental Botany v.v.iAcademy of Sciences of the Czech RepublicPrague 6Czech Republic
  2. 2.Department of Biology and WildlifeUniversity of Alaska at FairbanksFairbanksUSA
  3. 3.Institute of Arctic BiologyUniversity of Alaska at FairbanksFairbanksUSA

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