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Planta

, 228:319 | Cite as

Cloning and molecular characterisation of a potato SERK gene transcriptionally induced during initiation of somatic embryogenesis

  • Sanjeev Kumar Sharma
  • Steve Millam
  • Ingo Hein
  • Glenn J. Bryan
Original Article

Abstract

Somatic embryogenesis offers great potential in plant propagation, long-term germplasm conservation, and as a suitable model system for deciphering early events during embryogenesis. The up-regulation and ectopic expression of a SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) gene has been shown to mark and enhance embryogenic competence in somatic cells of model plant species. We have cloned and characterised a SERK gene (StSERK1) from potato (Solanum tuberosum L.), an important crop plant. Sequence analysis of StSERK1 revealed high levels of similarity to other plant SERKs, as well as a conserved intron/exon structure which is unique to members of the SERK family. Furthermore, StSERK clustered most closely with SERK gene family members such as MtSERK1, CuSERK1, AtSERK1, and DcSERK, implicated in evoking somatic embryogenesis. Monitoring of SERK expression during progression of potato somatic embryogenesis revealed increased StSERK expression during the induction phase. Subsequently, during the embryo transition phases, StSERK expression was unchanged and did not vary among embryo-forming and inhibitory conditions. However, in isolated somatic embryos StSERK expression was again up-regulated. In other plant parts (leaves, true potato seeds, microtubers and flower buds), StSERK showed different levels of expression. Expression analysis suggests that the isolated StSERK could be a functional SERK orthologue. The possible role of SERK as a marker of pluripotency, rather than embryogenesis alone, is discussed.

Keywords

Gene isolation Gene expression Leucine-rich repeat-receptor-like kinase (LRR-RLK) Mapping Solanum (potato) SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) gene StSERK 

Abbreviations

2,4-D

2,4 Dichlorophenoxyacetic acid

BAC

Bacterial artificial chromosome

EST

Expressed sequence tag

INS

Internodal segment

LRR

Leucine-rich repeat

ORF

Open reading frame

RLK

Receptor-like kinase

RT-PCR

Reverse transcription polymerase chain reaction

SE

Somatic embryogenesis

SERK

Somatic embryogenesis receptor-like kinase

SNP

Single nucleotide polymorphism

SPP

Serine-proline-proline

ZIP

Leucine zipper

Notes

Acknowledgements

S.K.S. is grateful to the Government of India and the Commonwealth Scholarship Commission, United Kingdom for his doctoral Commonwealth Scholarship Award. The authors are thankful to Drs Mark A. Taylor and Pete E. Hedley (SCRI) for critical reading of the manuscript. SCRI is supported by grant-in-aid from the Scottish Executive Environment & Rural Affairs Department (SEERAD).

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Sanjeev Kumar Sharma
    • 1
  • Steve Millam
    • 2
  • Ingo Hein
    • 1
  • Glenn J. Bryan
    • 1
  1. 1.Genetics Programme, Scottish Crop Research InstituteDundeeScotland, UK
  2. 2.Chichester CollegePulboroughUK

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