Planta

, Volume 227, Issue 5, pp 1181–1186 | Cite as

Incorporation and translocation of 2-deoxy-2-[18F]fluoro-d-glucose in Sorghum bicolor (L.) Moench monitored using a planar positron imaging system

  • Etsuko Hattori
  • Hiroshi Uchida
  • Norihiro Harada
  • Mari Ohta
  • Hideo Tsukada
  • Yasuhiro Hara
  • Tetsuya Suzuki
Rapid Communication

Abstract

[18F]FDG (2-deoxy-2-[18F]fluoro-d-glucose) was fed to a sorghum plant [Sorghum bicolor (L.) Moench] from the tip of a leaf and its movement was monitored using a planar positron imaging system (PPIS). [18F]FDG was uptaken from the leaf tip and it was translocated to the basal part of the shoots from where it moved to the roots, the tillers and the sheaths. Autoradiographic analysis of the distribution of 18F, [18F]FDG and/or its metabolites showed translocation to the roots, tillers, and to the leaves that were younger than the supplied leaf. Strong labelling was observed in the basal part of the shoots, in the sheaths, the youngest leaf and the root tips. Our results indicate that [18F]FDG and/or its metabolites were absorbed from the leaf and translocated to the sites where nutrients are required. This strongly suggests that [18F]FDG can be utilised as a tracer to study photoassimilate translocation in the living plant. This is the first report on the use of [18F]FDG, which is routinely used as a probe for clinical diagnosis, to study source to sink translocation of metabolites in whole plants in real time.

Keywords

2-Deoxy-2-[18F]fluoro-d-glucose Photoassimilate translocation Planar positron imaging system Sorghum 

Abbreviations

[18F]FDG

2-deoxy-2-[18F]fluoro-d-glucose

PPIS

Planar positron imaging system

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Etsuko Hattori
    • 1
    • 3
  • Hiroshi Uchida
    • 2
  • Norihiro Harada
    • 2
  • Mari Ohta
    • 3
  • Hideo Tsukada
    • 2
  • Yasuhiro Hara
    • 1
  • Tetsuya Suzuki
    • 3
    • 4
  1. 1.Bio Research Laboratory, Future Project DivisionToyota Motor CorporationToyotaJapan
  2. 2.Central Research LaboratoryHamamatsu Photonics K.KHamamatsuJapan
  3. 3.The Graduate School for the Creation of New Photonics IndustriesHamamatsuJapan
  4. 4.Faculty of Health Science, National Research Centre for Environmental ToxicologyThe University of QueenslandBrisbaneAustralia

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