Planta

, Volume 227, Issue 5, pp 1141–1150 | Cite as

Identification of an OsPR10a promoter region responsive to salicylic acid

  • Seon-Hee Hwang
  • In Ah Lee
  • Se Won Yie
  • Duk-Ju Hwang
Original Article

Abstract

Orysa sativa pathogenesis-related protein 10a (OsPR10a) was induced by pathogens, salicylic acid (SA), jasmonic acid (JA), ethephon, abscisic acid (ABA), and NaCl. We tried to analyze the OsPR10a promoter to investigate the transcriptional regulation of OsPR10a by SA. We demonstrated the inducibility of OsPR10a promoter by SA using transgenic Arabidopsis carrying OsPR10a:GFP as well as by transient expression assays in rice. To further identify the promoter region responsible for its induction by SA, four different deletions of the OsPR10a promoter were made, and their activities were measured by transient assays. The construct containing 687-bp OsPR10a promoter from its start codon exhibited a six-fold increase of induction compared to the control in response to SA. Mutation in the W-box like element 1 (WLE 1) between 687 and 637-bp from TGACA to TGAAA completely abolished induction of the OsPR10a promoter by SA, indicating that the WLE 1 between −687 and −637 of OsPR10a promoter is important in SA-mediated OsPR10a expression. We show for the first time that the W-box like element plays a role in SA mediated PR gene expression.

Keywords

cis-Acting element OsPR10a promoter Salicylic acid Salicylic acid induction 

Abbreviations

ABA

Abscisic acid

EREBP

Ethylene responsive element binding protein

ET

Ethylene

GA

Gibberellic acid

IAA

Indole acetic acid

JA

Jasmonic acid

NAA

Alpha-napthalene acetic acid

PR proteins

Pathogenesis-related proteins

SA

Salicylic acid

WLE

W-Box like element 1

Xoo

Xanthomonas oryzae pv oryzae

References

  1. Abe H, Yamaguchi-Shinizaki K, Urao T, Iwasaki T, Hosokawa D, Shinozaki K (1997) Role of Arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression. Plant Cell 9:1859–1868PubMedCrossRefGoogle Scholar
  2. Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gómez-Gómez L, Boller T, Ausubel FM, Sheen J (2002) MAP kinase signalling cascade in Arabidopsis innate immunity. Nature 415:977–983PubMedCrossRefGoogle Scholar
  3. Chen C, Chen Z (2002) Potentiation of developmentally regulated plant defense response by AtWRKY18, a pathogen-induced Arabidopsis transcription factor. Plant Physiol 129:706–716PubMedCrossRefGoogle Scholar
  4. Chen S, Tao L, Zeng L, Vega-Sanchez ME, Umemura K, Wang G-L (2006) A highly efficient transient protoplast system for analyzing defense gene expression and protein-protein interactions in rice. Mol Plant Pathol 7:417–427CrossRefGoogle Scholar
  5. Chern MS, Fitzgerald HA, Yadav RC, Canlas PE, Dong X, Ronald PC (2001) Evidence for a disease resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis. Plant J 27:101–113PubMedCrossRefGoogle Scholar
  6. Dempsey DA, Shah J, Klessig DF (1999) Salicylic acid and disease resistance in plants. Crit Rev Plant Sci 18:547–575CrossRefGoogle Scholar
  7. Eulgem T, Rushton PJ, Schmelzer E, Hahlbrock K, Somssich IE (1999) Early nuclear events in plant defence signalling: rapid gene activation by WRKY transcription factors. EMBO J 18:4689–4699PubMedCrossRefGoogle Scholar
  8. Fitzgerald HA, Canlas PE, Chern MS, Ronald PC (2005) Alteration of TGA factor activity in rice results in enhanced tolerance to Xanthomonas oryzae pv. oryzae. Plant J 43:335–347PubMedCrossRefGoogle Scholar
  9. Hashimoto M, Kisseleva L, Sawa S, Furukawa T, Komatsu S, Koshiba T (2004) A novel rice PR10 protein, RSOsPR10, specifically induced in roots by biotic and abiotic stresses, possibly via the jasmonic acid signaling pathway. Plant Cell Physiol 45:550–559PubMedCrossRefGoogle Scholar
  10. Heise A, Lippok B, Kirsh C, Hahlbrock K (2002) Two immediate-early pathogen-responsive members of the AtCMPG gene family in Arabidopsis thaliana and the W-box-containing elicitor-responsive element AtCMPG1. Proc Natl Acad Sci USA 99:9049–9054PubMedCrossRefGoogle Scholar
  11. Hong JK, Lee SC, Hwang BK (2005) Activation of pepper basic PR-1 gene promoter during defense signaling to pathogen, abiotic and environmental stresses. Gene 356:169–180PubMedCrossRefGoogle Scholar
  12. Johnson C, Boden E, Arias J (2003) Salicylic acid and NPR1 induce the recruitment of trans-activating TGA factors to a defense genes promoter in Arabidopsis. Plant Cell 15:1846–1858PubMedCrossRefGoogle Scholar
  13. Kagaya Y, Ohmiya K, Hattori T (1999) RAV1 a novel DNA-binding protein, binds to bipartite recognition sequence through two distinct DNA-binding domains uniquely found in higher plants. Nucleic Acids Res 27:470–478PubMedCrossRefGoogle Scholar
  14. Kirsch C, Logemann E, Lippok IB, Schmelzer E, Hahlbrock K (2001) A highly specific pathogen-responsive promoter element from the immediate-early activated CMPG1 gene in Petroselinum crispum. Plant J 26:217–227PubMedCrossRefGoogle Scholar
  15. Lee SC, Hwang BK (2006) Identification and deletion analysis of the promoter of the pepper SAR8.2 gene activated by bacterial infection and abiotic stresses. Planta 224:255–267PubMedCrossRefGoogle Scholar
  16. Li Y-F, Zhu R, Xu P (2005) Activation of the gene promoter of barley β-1,3-glucanase isoenzyme GIII is salicylic acid (SA)-dependent in transgenic rice plants. J Plant Res 118:215–221PubMedCrossRefGoogle Scholar
  17. Liu J-J, Ekramoddoullah AKM, Piggoyy N, Zamani A (2005a) Molecular cloning of a pathogen/wound-inducible PR10 promoter from Pinus monticola and characterization in transgenic Arabidopsis plants. Planta 221:159–169PubMedCrossRefGoogle Scholar
  18. Liu X, Bai X, Qian Q, Wang X, Chen M, Chu C (2005b) OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1. Cell Res 15:593–603PubMedCrossRefGoogle Scholar
  19. Liu X, Bai X, Wang X, Chu C (2006) OsWRKY71, a rice transcription factor, is involved in rice defense response. J Plant Physiol 164:969–979PubMedCrossRefGoogle Scholar
  20. Maleck K, Levine A, Eulgem T, Morgan A, Jürg S, Lawton KA, Dangle JL, Dietrich RA (2000) The transcriptome of Arabidopsis thaliana during systemic acquired resistance. Nat Genet 26:403–409PubMedCrossRefGoogle Scholar
  21. Malnoy M, Venisse J-S, Reynoird JP, Chevreau E (2003) Activation of three pathogen-inducible promoters of tobacco in transgenic pear (Pyrus communis L.) after abiotic and biotic elicitation. Planta 216:802–814PubMedGoogle Scholar
  22. Midoh N, Iwata M (1996) Cloning and characterization of a probenazole-inducible gene for an intracellular pathogenesis-related protein in rice. Plant Cell Physiol 37:9–18PubMedGoogle Scholar
  23. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–479CrossRefGoogle Scholar
  24. Pieterse CM, van Loon LC (1999) Salicylic acid-independent plant defense pathways. Trends Plant Sci 4:52–58PubMedCrossRefGoogle Scholar
  25. Rakwal R, Agrawal GK, Yonekura M (2001) Light-dependent induction of OsPR10 in rice (Oryza sativa L.) seedlings by the global stress signaling molecule jasmonic acid and protein phosphatase 2A inhibitors. Plant Sci 161:469–479CrossRefGoogle Scholar
  26. Robatzek S, Somssich IE (2001) A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence- and defense-related processes. Plant J 28:123–133PubMedCrossRefGoogle Scholar
  27. Rushton PJ, Torres JT, Parniske M, Wernert P, Hahlbrock K, Somssich IE (1996) Interaction of elicitor-induced DNA-binding proteins with elicitor response elements in the promoters of parsley PR1 genes. EMBO J 15:5690–5700PubMedGoogle Scholar
  28. Ruston PJ, Somssich IE (1998) Transcriptional control of plant genes responsive to pathogens. Curr Opin Plant Biol 1:311–315CrossRefGoogle Scholar
  29. Rushton PJ, Reinstadler A, Lipka V, Lippok B, Somssich IE (2002) Synthetic plant promoters containing defined regulatory elements provides novel insights into pathogen- and wound-induced signaling. Plant Cell 14:749–762PubMedCrossRefGoogle Scholar
  30. Ryu HS, Han M, Lee SK, Cho JI, Ryoo N, Heu S, Lee YH, Bhoo SH, Wang G, Hahn TR, Jeon JS (2006) A comprehensive expression analysis of the WRKY gene superfamily in rice plants during defense response. Plant Cell Rep 25:836–847PubMedCrossRefGoogle Scholar
  31. Sela-Buurlage MB, Ponstein AS, Bres-Vloemans SA, Melchers LS, Van den Elzen PJM, Cornelissen BJC (1993) Only specific tobacco (Nicotiana tabacum) chitinases and β-1, 3-glucanases exhibit antifungal activity. Plant Physiol 101:857–863PubMedGoogle Scholar
  32. Shimono M, Sugano S, Nakayama A, Jiang C-J, Ono K, Toki S, Takatsuji H (2007) Rice WRKY45 plays a crucial role in benzothiadiazole-inducible blast resistance. Plant Cell 19:2064–2076PubMedCrossRefGoogle Scholar
  33. Shinozaki K, Yamaguchi-Shinozaki (1996) Molecular responses to drought and cold stress. Curr Opin Biotechnol 7:161–167PubMedCrossRefGoogle Scholar
  34. Shinshi H, Usami S, Ohme-Takagi M (1995) Identification of an ethylene-responsive region in the promoter of a tobacco class I chitinase gene. Plant Mol Biol 27:923–932PubMedCrossRefGoogle Scholar
  35. Singh K, Foley RC, Onate-Sanchez L (2002) Transcription factors in plant defense and stress responses. Curr Opin Plant Biol 5:430–436PubMedCrossRefGoogle Scholar
  36. Sohn KH, Lee SC, Jung HW, Hong JK, Hwang BK (2006) Overexpression of the pepper CARAV1 pathogen-induced gene encoding a RAV transcription factor induces pathogenesis-related genes and enhances resistance to bacterial pathogen in Arabidopsis. Plant Mol Biol 61:897–915PubMedCrossRefGoogle Scholar
  37. Van Loon LC, Van Strien EA (1999) The families of pathogenesis-related proteins, their activities, and comprehensive analysis of PR-1 type proteins. Physiol Mol Plant Pathol 55:65–97CrossRefGoogle Scholar
  38. Woloshuk CP, Meulenhoff JS, Sela-Buurlage M, Van den Elyzen PJM, Cornelissen BJC (1991) Pathogen-induced proteins with inhibitory activity toward Phytophthora infestans. Plant Cell 3:619–628PubMedCrossRefGoogle Scholar
  39. Yamamoto S, Nakano T, Suzuki K, Shinshi H (2004) Elicitor-induced activation of transcription via W box-related cis-acting elements from a basic chitinase gene by WRKY transcription factors in tobacco. Biochim Biophys Acta 1679:279–287PubMedGoogle Scholar
  40. Yu D, Chen C, Chen Z (2001) Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression. Plant Cell 13:1527–1540PubMedCrossRefGoogle Scholar

Copyright information

© The Author(s) 2008

Authors and Affiliations

  • Seon-Hee Hwang
    • 1
    • 2
  • In Ah Lee
    • 1
  • Se Won Yie
    • 2
  • Duk-Ju Hwang
    • 1
  1. 1.National Institute of Agricultural BiotechnologyRural Development AdministrationSuwonSouth Korea
  2. 2.Department of Molecular Bioscience, School of Biosciences and BiotechnologyKangwon National UniversityChuncheonSouth Korea

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