, Volume 227, Issue 5, pp 1141–1150 | Cite as

Identification of an OsPR10a promoter region responsive to salicylic acid

  • Seon-Hee Hwang
  • In Ah Lee
  • Se Won Yie
  • Duk-Ju Hwang
Original Article


Orysa sativa pathogenesis-related protein 10a (OsPR10a) was induced by pathogens, salicylic acid (SA), jasmonic acid (JA), ethephon, abscisic acid (ABA), and NaCl. We tried to analyze the OsPR10a promoter to investigate the transcriptional regulation of OsPR10a by SA. We demonstrated the inducibility of OsPR10a promoter by SA using transgenic Arabidopsis carrying OsPR10a:GFP as well as by transient expression assays in rice. To further identify the promoter region responsible for its induction by SA, four different deletions of the OsPR10a promoter were made, and their activities were measured by transient assays. The construct containing 687-bp OsPR10a promoter from its start codon exhibited a six-fold increase of induction compared to the control in response to SA. Mutation in the W-box like element 1 (WLE 1) between 687 and 637-bp from TGACA to TGAAA completely abolished induction of the OsPR10a promoter by SA, indicating that the WLE 1 between −687 and −637 of OsPR10a promoter is important in SA-mediated OsPR10a expression. We show for the first time that the W-box like element plays a role in SA mediated PR gene expression.


cis-Acting element OsPR10a promoter Salicylic acid Salicylic acid induction 



Abscisic acid


Ethylene responsive element binding protein




Gibberellic acid


Indole acetic acid


Jasmonic acid


Alpha-napthalene acetic acid

PR proteins

Pathogenesis-related proteins


Salicylic acid


W-Box like element 1


Xanthomonas oryzae pv oryzae


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Copyright information

© The Author(s) 2008

Authors and Affiliations

  • Seon-Hee Hwang
    • 1
    • 2
  • In Ah Lee
    • 1
  • Se Won Yie
    • 2
  • Duk-Ju Hwang
    • 1
  1. 1.National Institute of Agricultural BiotechnologyRural Development AdministrationSuwonSouth Korea
  2. 2.Department of Molecular Bioscience, School of Biosciences and BiotechnologyKangwon National UniversityChuncheonSouth Korea

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