Planta

, Volume 227, Issue 5, pp 1063–1077

Carbohydrate mobilization and gene regulatory profile in the pseudobulb of Oncidium orchid during the flowering process

  • Chih-Yu Wang
  • Chung-Yi Chiou
  • Heng-Long Wang
  • Ramanarayan Krishnamurthy
  • Shripathi Venkatagiri
  • Jun Tan
  • Kai-Wun Yeh
Original Article

Abstract

The pseudobulb of Oncidium orchid is a storage organ for supplying water, minerals and carbohydrates to the developing inflorescence. Different patterns of mannan, starch and pectin metabolism were observed in the pseudobulb of three developmental stages by histochemical staining and high performance anion exchange chromatographic (HPAEC) analysis. Copious pectin was strongly stained by ruthenium red in young pseudobulbs demonstrating that mannan and pectin were preferentially accumulated in the young pseudobulb sink at inflorescence pre-initiation stage. Concomitant with the emergence of the inflorescence, mannan and pectin decreased gradually and converted to starch. The starch, synthesized at the inflorescence developing stage, was eventually degraded at the floral development stage. A systematic survey on the subtractive EST (expression sequence tag) library of pseudobulb in the inflorescence pre-initiation stage revealed the presence of five groups of gene homologues related to sucrose, mannan, starch, pectin and other carbohydrate metabolism. The transcriptional level of 13 relevant genes related to carbohydrate metabolism was characterized from pseudobulbs of three different developmental stages. The specific activities of the enzymes encoded by these genes were also assayed. The expression profiles of these genes show that the transcriptional levels largely correlated with the enzyme activities, which were associated with the respective carbohydrate pools. These results demonstrated a novel functional profile of polysaccharide mobilization pathway as well as their relevant gene expression in the pseudobulb of Oncidium orchid during the flowering process.

Keywords

Carbohydrate metabolism EST (expressed sequence tags) Inflorescence Oncidium Pseudobulb 

Abbreviations

HPAEC

High performance anion exchange chromatography

EST

Expression sequence tags

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Chih-Yu Wang
    • 1
  • Chung-Yi Chiou
    • 1
  • Heng-Long Wang
    • 2
  • Ramanarayan Krishnamurthy
    • 1
  • Shripathi Venkatagiri
    • 4
  • Jun Tan
    • 1
    • 3
  • Kai-Wun Yeh
    • 1
  1. 1.Institute of Plant BiologyNational Taiwan UniversityTaipei, 106Taiwan
  2. 2.Department of Life ScienceNational University of KaohsiungKaohsiungTaiwan
  3. 3.College of BioinformaticsChongqing University of Posts and TelecommunicationsChongqingChina
  4. 4.Department of BotanyKarnatak UniversityDharwadIndia

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