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Planta

, Volume 227, Issue 3, pp 641–647 | Cite as

Cation dependent O-methyltransferases from rice

  • Yoon Jung Lee
  • Bong Gyu Kim
  • Youhoon Chong
  • Yoongho Lim
  • Joong-Hoon AhnEmail author
Original Article

Abstract

Two lower molecular mass OMT genes (ROMT-15 and -17) were cloned from rice and expressed in Escherichia coli as glutathione S-transferase fusion proteins. ROMT-15 and -17 metabolized caffeoyl-CoA, flavones and flavonols containing two vicinal hydroxyl groups, although they exhibited different substrate specificities. The position of methylation in both luteolin and quercetin was determined to be the 3′ hydroxyl group and myricetin and tricetin were methylated not only at 3′ but also at 5′ hydroxyl groups. ROMT-15 and -17 are cation-dependent and mutation of the predicted metal binding sites resulted in the loss of the enzyme activity, indicating that the metal ion has a critical role in the enzymatic methylation.

Keywords

Caffeoyl-CoA Flavonoid O-methyltransferase Oryza sativa 

Abbreviations

AdoMet

S-adenosyl methionine

CCoA

Caffeoyl-CoA

CCoAOMT

Caffeoyl coenzyme A OMT

GST

Gluthathione S-transferase

5HFA

5-Hydroxyferulic acid

OMT

O-methyltransferase

Notes

Acknowledgments

This work was supported by a grant from the Biogreen 21 Program, Rural Development Administration, Republic of Korea and partially by grant KRF-2006-005-J03401and the second Brain Korea 21 (Ministry of Education).

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Yoon Jung Lee
    • 1
  • Bong Gyu Kim
    • 1
  • Youhoon Chong
    • 1
  • Yoongho Lim
    • 1
  • Joong-Hoon Ahn
    • 1
    Email author
  1. 1.Department of Bioscience and Biotechnology, Bio/Molecular Informatics CenterKonkuk UniversitySeoulSouth Korea

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